Evaluation and Comparison of Antioxidant Activity of Urtica Dioica L. Leaf and Stem by MAE

Lohrasbi Dashtaki, M. R. (2013) Evaluation and Comparison of Antioxidant Activity of Urtica Dioica L. Leaf and Stem by MAE. Masters thesis, University of Zabol.

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Medicinal Herbs are rich in phenolic compounds, which are considered most important natural antioxidants. In the present study, the antioxidant activity of medicinal plant extracts of Urtica Dioica L. aerial parts were evaluated and compared. Therefore, the microwave-assisted extraction and traditional methods were performed. In order to optimize the microwave extraction conditions, the parameters affecting the extraction efficiency such as instrument power (200-600 W), the type of extraction solvent (water, ethanol and methanol), extraction temperature (60-110 °C) and time (2-20 min) were investigated. For traditional extraction herb powder in the presence of solvent (water, ethanol and methanol) for 48 hours were placed on the electric mixer. To evaluate the antioxidant activity, extract in 1:1 to 2, 2-diphenyl-1-picrylhydrazyl (DPPH) chemical reagent was added and placed in a dark place. Then, the solution to high performance liquid chromatography (HPLC) was injected. Progress of the reaction between the extract and represent reagent, the amount of antioxidant activity was determined. Finally, the results were analyzed by SPSS software. This research showed that the antioxidant activity of extract of leave is more than the stem.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Urtica Dioica L; Antioxidant Activity; Microwave Assisted Extraction ; High Performance Liquid Chromatography ; DPPH
Subjects: Q Science > QD Chemistry
S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 14 May 2016 08:27
Last Modified: 14 May 2016 08:27
URI: http://eprints.uoz.ac.ir/id/eprint/575

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