Detection of SCGB2A2 expression in breast cancer tissue with Real-time Reverse Transcriptase Polymerase Chain Reaction

Akhtardanesh, M. (2013) Detection of SCGB2A2 expression in breast cancer tissue with Real-time Reverse Transcriptase Polymerase Chain Reaction. Masters thesis, University of Zabol.

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In many countries, including developed countries, breast cancer is the most common cancer in women. In Iran, breast cancer with frequency of 15/9 is the most common malignancy and the attacked age is 10 years less than in Western countries. New statistics reports comparing with previous reports statistics indicate a relatively rapid increase in the incidence of the disease in Iranian women. Great majority of human breast cancers arise from epithelial cell that surround lobular and ductal mammary gland. The evolution of breast cancer is accompanied by multiple genetic changes that result in qualitative and quantitative alterations in individual gene expression. Many of these quantitative genetic changes manifest themselves as alterations in the cellular complement of novel transcribed mRNAs. Identification of these mRNAs, if sufficiently characterized, could provide clinically useful information for patient management and prognosis. And also enhance our understanding of breast cancer pathogenesis. SCGB2A2 is one of the newest genes that alteration of its expression in breast tumor tissue has been reported in recent year's research. The function of this gene is unknown, related family members, epithelial secretory proteins that can either modulate inflammatory processes or bind steroid ligands, are small. According to the importance and necessity of studing breast cancer patient, in this research we analyzed the expression of SCGB2A2 genes in breast cancer patient of Sistan and Balouchestan, Bam and Kerman. The data was collected from twenty-two control samples and seventeen samples of breast cancer tumour. Then, to study the qualitative and quantitative SCGB2A2 gene expression of collected tissues, RNA was extracted from all samples and expression analysed performed by real time PCR method. Data showed significant difference in SCGB2A2 mRNA gene expressions among normal tissue and tumor ones. The results indicated that the expression of SCGB2A2 gene in tumor breast samples is more than the control groups. We did not find any significant differences in SCGB2A2 mRNA expressions in grade III tumors in contrary with GradeI and Grade II tumors.

Item Type: Thesis (Masters)
Uncontrolled Keywords: breast cancer, gene expression, Real-Time RT PCR, tumor marker, SCGB2A2
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: admin admin1 admin2
Date Deposited: 24 Apr 2016 04:21
Last Modified: 24 Apr 2016 04:21

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