Association study of promoter polymorphism of the CYP27B1 gene with multiple sclerosis patients in East of Iran

Hosseini, S. (2014) Association study of promoter polymorphism of the CYP27B1 gene with multiple sclerosis patients in East of Iran. Masters thesis, University of Zabol.

[img]
Preview
 Text
Association study of promoter polymorphism of the CYP27B1 gene.pdf
Download (241kB) | Preview

Abstract

Multiple sclerosis (MS) is a complex disease in the central nervous system (CNS) and the most common inflammatory demyelinating and degenerative disease in young adults. Many studies have shown that, vitamin D may interfere with the pathophysiology of MS within the CNS. Vitamin D does not only interfere with immune responses of the periphery, but it can play a role in regulating the neurodegeneration and repair processes within the CNS. CYP27B1 gene encodes the enzyme that catalyses the formation of the biological active metabolite 1, 25(OH) 2D and form 25(OH) D of the abundant metabolite. CYP27B1 is expressed on a variety of cells including neurons, glial cells, and invaded lymphocyte. We analyzed the polymorphism profiling of CYP27B1 gene in rs703842 SNP among 209 MS patients and 221 age_matched healthy individual controls in the east of Iran. DNA was extracted from whole blood using the boiling method. The rs703842 was genotyped, applying Rsa restriction enzyme. SPSS version 19 was used to analyze the data. Genotypic and allelic frequencies were compared between patients and controls using the Chi-Square Tests. We investigated the genotypic and allelic analyses of this risk SNP but did not find any association with complete MS cohort, but the stratification of gender and MS subtype analyses were showed significantly associated with genotyping in MS (p>0.05).

Item Type: Thesis (Masters)
Uncontrolled Keywords: polymorphism, CYP27B1, multiple sclerosis, PCR-RFLP
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: admin admin1 admin2
Date Deposited: 24 Apr 2016 04:18
Last Modified: 24 Apr 2016 04:18
URI: http://eprints.uoz.ac.ir/id/eprint/474

Actions (login required)

View Item View Item
Uoz is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.