Cloning of Mycobacterium tuberculosis Major Secreted Protein Antigen 85B (Ag85B) in Escherichia coli

Purghasemy Fatideh, S. (2014) Cloning of Mycobacterium tuberculosis Major Secreted Protein Antigen 85B (Ag85B) in Escherichia coli. Masters thesis, University of Zabol.

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Altough BCG vaccine is a live vaccine, that produced and dropped virulence of pathogenic microorganisms, it is weak in Iran and acts as a antigens to develop immunity. It prepared continuous culturing mycobacterium bovis BCG vaccine, which has been growing for 13 years in the bile (1921- 1908). The vaccine's protective acts against tuberculosis, or TB. But the hazards and obstructions such as incidence of tuberculous meningitis vaccine for infants as well as in some vaccines is contraindicated in patients with immune deficiency. Theoretically, any protein-coding genes by recombinant techniques in bacteria, yeast, or mammalian cells, can be used for replication and expression. In this regard, the major protein of 30 kDa, Ag85B of Mycobacterium tuberculosis was selected as the first TB vaccine candidate. The secretory antigen that is able to stimulate an immune response modifies Hmchnnyn Prevention and INF-γ production in the animal models. The aim of this study was to isolate the gene encoding surface antigen 85B of Mycobacterium tuberculosis strains H37Rv and cloned the gene encoding surface antigen 85B and transferred it to a plasmid without any change in the nucleotide structure of it. Genes that were responsible for the secretion of this protein were cloned into the plasmid PCAMBIA 1305.2. To produce recombinant antigen 85B gene was amplified by PCR and then cloned by fbpB and transferred by the process of the E.coli strain DH5α.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Mycobacterium tuberculosis ،Antigen 85B ، Cloning
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: admin admin1 admin2
Date Deposited: 23 Apr 2016 08:23
Last Modified: 23 Apr 2016 08:23

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