Antioxidant activity evaluation of Mentha pulegium extract using MAE

Soozangar, Arash (2012) Antioxidant activity evaluation of Mentha pulegium extract using MAE. Masters thesis, University of Zabol.

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The significance of oxidation in the body and in foodstuffs has been widely recognized. Oxidative metabolism is essential for the survival of cells. A side effect of this dependence is the production of free radicals and other reactive oxygen species that cause oxidative changes. Oxidation can also effect foods, it is one of the major causes of chemical spoilage, resulting in rancidity or deterioration of the nutritional quality, colour, flavor, texture and safety of foods. Altough, oxidation of food can be prevented by synthetic antioxidants including butylated hydroxyanisole (BHA), butylated hydroxy toluene (BHT) and propyl gallate (PG), but their safety has been questioned. Hence, there is a need to identify new natural antioxidants for prevention of lipid peroxidation in the food industry. Th eaim of this study was to evaluate antioxidant activity of Mentha pulegium (Pennyroyal) extracted by DPPH assay and compare the extracted microwave method with the traditional extraction. Mentha pulegium antioxidant activities was determined Using (2,2 Diphenyl-1-picrylhydrazyl) DPPH method based on Subtracting peak area of HPLC chromatograms. In microwave method 0.5 gr of plant in 10 ml ethanol, methanol and water solvents was used. After finding the optimal solvent type optimized Power microwave devices was used for time and temperature extraction. The extraction from microwave and traditional methods were compared. The microwave extract showed better efficiency for scavenging free radicals. SPSS version 15 was used for anlysiis th data. No significant statistically differences was found between microwave and traditional extracts. So extract active components with GC-MS method was recommended.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Antioxidant activity- Mentha pulegium- MAE- DPPH
Subjects: Q Science > QD Chemistry
Depositing User: admin admin1 admin2
Date Deposited: 23 Apr 2016 08:15
Last Modified: 23 Apr 2016 08:15

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