Tohidi, A. (2011) Evaluation of GSK gene expression in selected tritipyrum lines using Real Time PCR. Masters thesis, University of Zabol.
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Abstract
In order to determine TaGSK1 gene expression levels in 12 selected tritipyrum genotypes and 2 wheat genotypes, a comparison between genotypes were performed under control and stress conditions in Zabol University Biotechnology Institute. Tree replicates for each genotype was considered and then specimens under stress were subjected NaCl 200 mM. RNA was extracted from leaf samples. cDNA was designed using reverse transcription. 18S rRNA gene expression was used as internal control gene in Real Time PCR to normalize TaGSK1 gene expression levels. Results showed St/b genotype had significant differences between level of TaGSK1 gene expression under conditions of salinity and control.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | TaGSK1 gene, Gene expression, Salt stress, Tritipyrum and Real Time PCR |
| Subjects: | Q Science > QH Natural history > QH426 Genetics S Agriculture > SB Plant culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 23 Apr 2016 05:00 |
| Last Modified: | 23 Apr 2016 05:00 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/436 |
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