Effect of Hydro-Alcoholic Prosopis farcta fruit extract on Gene Expression of Gluco Kinase in Diabetic rats Type (1)

Noora, Habib (2014) Effect of Hydro-Alcoholic Prosopis farcta fruit extract on Gene Expression of Gluco Kinase in Diabetic rats Type (1). Masters thesis, University of Zabol.

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Diabetes mellitus is one of the metabolic diseases in which it associate with relative or absolute lack of Insulin, increase in blood glucose level and disorder in the metabolism of the carbohydrates, fats and proteins. Many of plants are effective for the treatment control and prevention of diabetes. Here we focused on Prosopis farcta plant on diabetes treatment. Glycokinase enzyme causes facilitating of glucose Phosphorilation and changing to glucose 6-phosphate thus it has key role in the using of glucose. The purpose of the study is the effect of Hydroalcoholic extract of Prosopis farcta fruit on Glucokinaze gen expression in diabetic rats. For this reason, 45 male Wistar rats with 150-200 gr weight was selected, and they was divided into a groups of 15 rats: Healthy controls, diabetic control and treated diabetic rats. With injection of rats by intraperitoneal procedure, the rats addicted to diabetes. The treated diabetic rats nourished daily 300 mg/kg of Prosopis farcta fruit extract by gavage method for 30 days. The healthy controls and diabetic controls nourished with Drstilled Water, then the day before injection of extract, day 15 and day 30 after injection the blood glucose level measured. Based on evidence to on day 15 level of the blood glucose level decreased in treated diabetic rats in comparison with diabetic controls. And in day 30 there was no different between them. With Real-Time PCR method on liver tissue, the amount of Glucokinase gen expression analyzed. So results show that the F test is not significant and there is not any significant different between out of nine.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Prosopis farcta, Diabetes mellitus type 1, Glucokinase, Real Time PCR
Subjects: Q Science > QH Natural history > QH426 Genetics
S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 20 Apr 2016 05:59
Last Modified: 20 Apr 2016 05:59
URI: http://eprints.uoz.ac.ir/id/eprint/414

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