Effect of Hibiscus Sabdariffa on Performance, immune response, and Biochemical Parameters in Broiler Chickens fed Normal or Aflatoxin Contaminated Diets

Mohammadi, F. (2014) Effect of Hibiscus Sabdariffa on Performance, immune response, and Biochemical Parameters in Broiler Chickens fed Normal or Aflatoxin Contaminated Diets. Masters thesis, University of Zabol.

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Abstract

This experiment was conducted to evaluate the inhibitory ability of Roselle (Hibiscus Sabdariffa) against aflatoxin B1 (AFB1). A total of 192 one-day-old broiler chicks were divided into four experimental groups: without Roselle-without aflatoxin B1 (Control), without Roselle and with aflatoxin B1 (AFB1 group), with Roselle-without aflatoxin B1 (ROS group), with Roselle-with aflatoxin (AFB1-ROS group). Ten gram of Roselle per kilogram of diet was added to Roselle receiving groups and AFB1- treatments received 2.5 milligram of AFB1 per kilogram of diet. The effect of various treatments on feed intake was significant (P < 0.05) in which the lowest and highest feed intake were seen in AFB1 and ROS groups, respectively. Body weight gain did not differ between AFB1-ROS and control groups (P > 0.05). Feed conversion ratio was not significantly affected by neither aflatoxin nor Roselle administrations (P > 0.05). However, AFB1 group had a higher feed conversion ratio than other groups (P < 0.05). The lowest relative weight of heart was observed in the AFB1 group but the highest and lowest relative weight of bursa of Fabricious belonged to ROS and AFB1 groups, respectively (P > 0.05). The highest and lowest relative weights of liver were observed in AFB1and ROS groups, respectively. The activities of lactate-dehydronage (LDH), gamma-glutamyl B transferees (GGT), aspartat amino transferees (AST), Alanin amino transferees (ALT) and alkaline phosphates (ALP) enzymes in AFB1 group were higher than other groups (P < 0.05). The antibody titer against Newcastle virus disease was lower in AFB1 group than the ROS group (P < 0.05). The highest antibody titer against sheep red blood cells (SRBC) belonged to ROS group and followed by AFB1-ROS groups and control. There was no significant effect of different treatments on Haematocrit percent (P > 0.05). Increase in skin thickness of challenged birds with 2, 4-dinitrochlorobenzene (DNCB) was lower in AFB1 group than other groups (P < 0.05) but the most skin thickness was seen in ROS group. The ash contents of toe and shank were significantly different among treatments (P < 0.05) in which the highest ash contents of toe and shank belonged to ROS group. The highest and lowest amount of malondialdehyde (MDA) in fresh and frozen meat samples was observed in AFB1 and ROS groups, respectively (P < 0.05). The E. coli population in ileum contents was increased in AFB1 group (P < 0.05) while their colonies decreased by the use of Roselle in the diet (P< 0.05). In contrast, the use of Roselle in the diet increased the lactic acid bacteria in the ileum contents (P < 0.05).

Item Type: Thesis (Masters)
Uncontrolled Keywords: Broiler chicken, Aflatoxicosis, Hibiscus Sabdariffa, Performance, Immunity, Liver enzymes
Subjects: S Agriculture > SF Animal culture
Depositing User: admin admin1 admin2
Date Deposited: 20 Apr 2016 05:44
Last Modified: 20 Apr 2016 05:44
URI: http://eprints.uoz.ac.ir/id/eprint/411

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