Nabavi, Yasin (2024) Clinical and histopathological study of local effects of Aloe vera and Alhagi maurorum extracts on open wounds in rats. Doctoral thesis, university of zabol.
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Clinical and histopathological study of local effects of Aloe vera and Alhagi maurorum extracts on open wounds in rats.pdf Download (258kB) | Preview |
Abstract
Introduction: A wound is defined as a disruption in the normal continuity of the skin or mucous membranes that can be acute or chronic. Acute wounds typically heal within the expected time, while chronic wounds do not heal properly due to infection or health conditions. The global approach to herbal medicine has increased due to advantages such as fewer side effects and the World Health Organization's emphasis on the use of medicinal plants. Aloe vera is of interest as a wound healing treatment due to its anti-inflammatory and moisturizing properties. Camellia sinensis is also effective in the treatment of stomach ulcers and oral ulcers. Considering the progress of modern methods in wound healing on the one hand and the desire to use the healing properties of medicinal plants as complementary medicine on the other hand, as well as the mentioned properties of Aloe vera and yarrow in better and faster wound healing, in this study we decided to clinically and histopathologically investigate the local effects of Aloe vera and yarrow plant extracts on open wounds in rats. Method and material: In this study, 100 Wistar rats were divided into five experimental groups. Open wounds were created on the back of the rats to evaluate the effects of the treatments, and then the rats were divided into five different groups to receive specific treatments. Each group consisted of 20 rats. Group A was treated with 10% itch-killer, Group B was treated with 10% aloe vera, and Group C was treated with a combination of both substances. Group X received tetracycline ointment as a treatment control group, while Group O received only the ointment base as a positive control. After anesthesia, the rats were prepared for surgery. At the end of the experimental period, pathology samples were collected from the wound sites and the data obtained from the photographs and samples were reviewed to analyze the effects of the treatments. Digimizer software was used to perform macroscopic measurements in this study. With this software, the total area of the wound in each image was measured. Two separate methods were used for microscopic examination. The first method was the Adam et al. scoring system method and the second method was the researcher-made scoring system. The collected data were analyzed with SPSS version 27 software and the results were obtained. The significance level in this study was 0.05. Results: The results showed that macroscopically there was no significant difference between the studied groups in terms of wound survival percentage. Microscopically, among all the factors examined, the subgroups of "mitotic divisions of basal cells", "proliferation of spiny cells", "differentiation of spiny cells", "granular cells", "epidermal formation rate", "direction of blood vessels" and "hair follicles" were identified as significant factors with a P value less than 0.05. In these parameters, the scores of the 10% itch and 10% aloe vera groups were often significantly higher than the positive control groups. Conclusion: The results of our study showed that there was no significant difference in the wound healing process between the treatment groups (Aloe vera, stinging nettle, and a combination of both) compared to the control group in the macroscopic section and there was a significant difference in the macroscopic section. Microscopic examination showed that there were positive effects in certain subgroups such as "mitotic divisions" and "proliferation of spiny cells" and ... indicating the positive effect of these two plants on the wound healing process. , But for the effective use of these substances in the treatment of wounds, more research is needed to better understand the mechanisms of action and determine the optimal doses.
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | Aloe vera, open wound, rat, Alhagi maurorum |
| Subjects: | S Agriculture > SF Animal culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 18 May 2025 05:38 |
| Last Modified: | 18 May 2025 05:38 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3818 |
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