Kord, Abdolkhalegh (2024) The effect of ascorbic acid on the agronomic and biochemical traits of Plantago ovata L. under drought non-stress and stress conditions in green house and biochemical traits of produced calli from it. Masters thesis, University of Zabol.
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The effect of ascorbic acid on the agronomic and biochemical traits of Plantago ovata L. under drought non-stress and stress conditions in green house and biochemical traits of produced calli from it.pdf Download (53kB) | Preview |
Abstract
Plantago ovata L. is one of the most important species of the plantaginaceae family. In this study, the effect of ascorbic acid and moisture stress on the agronomic and biochemical traits and antioxidant activity of the seeds in the greenhouse and the effect of ascorbic acid on the biochemical characteristics of the calluses obtained from this plant was evaluated. The studied treatments in the greenhouse were four irrigation regimes (100, 75, 50 and 25% of field capacity) and four different concentrations of ascorbic acid including 0, 5, 10 and 20 mM. Four different concentrations of ascorbic acid including 0, 250, 500 and 1000 mg/l was used in the callus cultures. Agronomic and biochemical traits and antioxidant activity as a completely randomized block design with three replications in a greenhouse located in Sangan region of Khash city and biochemical traits and antioxidant activity of callus as a completely randomized design with three replications in the central laboratory of Zabul University were performed. The results showed that irrigation regime, ascorbic acid and their interaction were significant on most of the measured traits in the greenhouse. Moisture stress led to a decrease in plant height, biological yield, seed yield and its components, and harvested index, while it caused an increase in mucilage content, total phenol and flavonoid contents and antioxidant activity of the seed. The effect of drought stress on these traits depended on the level of ascorbic acid and the intensity of the stress. Ascorbic acid led to the improvement of most of the traits in both water non-stress and stress conditions, and the amount of increase of these traits depended on the level of irrigation regime and the concentration of ascorbic acid. Usually, at its highest concentration (20 mM), it had a better effect compared to other concentrations. In callus cultures, its lower concentration (250 mg/liter) led to a higher increase in total phenol and flavonoid contents and antioxidant activity. The seed yield was the highest under water non-stress condition and foliar spraying of plants with a concentration of 20 mM of ascorbic acid (2.69 g/plant) and the lowest value was shown under severe water stress condition and no foliar spraying of plants with ascorbic acid. (1.04 g/plantTherefore, callus culture can be used as an effective technique to produce effective compounds throughout the year and out of natural limits. Phenolic and flavonoid compounds of seed and callus were identified by HPLC analysis. The effect of moisture stress and ascorbic acid on these compounds depended on the intensity of stress, different concentrations of ascorbic acid and the type of compound. Therefore, their effects were different and some compounds increased and some of them decreased. In relation to callus compounds, the effect of ascorbic acid on them was also different). Therefore, ascorbic acid can be considered as an effective antioxidant in improving the yield and effective compounds of seeds in water non-stress and stress conditiona and increasing the secondary metabolites of callus. The amounts of effective compounds in callus were close to their amounts in seeds obtained under greenhouse condition..
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | ascorbic acid, callus culture, moisture stress, Plantago ovata L., secondary metabolites |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 04 May 2025 07:07 |
| Last Modified: | 04 May 2025 07:07 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3816 |
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