Azar, M. (2024) Effect of mycorrhizal fungi and Fe3o4 nanoparticles on salt stress tolerance of Lepidium sativumum. Masters thesis, University of Zabol.
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Effect of mycorrhizal fungi and Fe3o4 nanoparticles on salt stress tolerance of Lepidium sativumum.pdf Download (114kB) | Preview |
Abstract
Watercress (Lepidium sativum), an annual plant from Brassicaceae family, is an appetizer, blood purifier and blood vessel opener. In this research, the effects of different concentrations of salinity stress, iron oxide nanoparticles, and mycorrhizal fungi on growth and physiological traits of watercress were investigated. This factorial experiment was conducted based on a completely randomized design with 13 treatment levels in triplicate. Cultivation was done in plastic pots with the following soil mixture: peat moss: soil: perlite (1:1:1), and then the soil mixture was blended with two mycorrhizal fungi species, namely G.intraradices and G.mosseae separately at 1:9 ratio. Plants were three times sprayed with iron oxide nanoparticles at four-leaf stage (21 days after cultivation) every other day. One day after the treatment, the salinity stress was five times applied in the form of solutions with 0, 40 and 80 mM concentrations every other day, and then morphological traits such as the length and weight of shoot and root, biochemical indices such as the content of photosynthetic pigments, proline, protein, phenol, flavonoid, antioxidant activity. (DPPH), lipid peroxidation (MDA) and the activity of antioxidant enzymes (guaiacol peroxidase and polyphenol oxidase) were measured by spectrophotometric method and statistical analysis was performed using SPSS software (version 27). The results showed that salinity stress at a concentration of 80 mM decreased length and weight of roots and shoots, the amount of chlorophyll a, b, total chlorophyll, carotenoid and soluble protein compared to the control, but the combined treatment of mycorrhizal fungi and iron nanoparticles improved these indicators, especially at 80 mM salinity compared to treatment with fungi alone. Salinity stress increased the amount of proline, content of malondaldehyde (MDA), phenol, flavonoid, antioxidant activity (DPPH) and activity of antioxidant enzymes compared to the control. The combined treatment of mycorrhizal fungus and iron nanoparticle increased these indicators (except malonaldehyde), especially at 80 mM salinity, compared to the treatment of fungus alone. The results of the present study showed that G. mosseae is more effective than G. intraradices. Based on the results of this research, the combined treatment of iron nanoparticles and mycorrhizal fungus G. mosseae is suggested to reduce the harmful effects of salinity stress.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Watercress, Phenolic Compounds, Iron Oxide Nanoparticles, Mycorrhizal Fungus, Salinity Stress |
| Subjects: | Q Science > QK Botany |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 08 Mar 2025 06:39 |
| Last Modified: | 08 Mar 2025 06:39 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3774 |
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