Saadatian, Rezvaneh (2024) The Prevalence of Sea and Seb Genes in the Production of Enterotoxins A and B in Staphylococcus Aureus Bacteria in Sistan Province. Masters thesis, University of Zabol.
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The Prevalence of Sea and Seb Genes in the Production of Enterotoxins A and B in Staphylococcus Aureus Bacteria in Sistan Province.pdf Download (382kB) |
Abstract
Staphylococcus aureus is one of the most important human pathogens that produces a wide range of toxins and causes various diseases. Enterotoxins A and B are the main causes of food poisoning in the world. The aim of this research was to investigate enterotoxin A (sea) and B (seb) genes in S. aureus isolated from raw milk of traditional farms in Sistan region. The raw milk samples (n=300) were sent to the laboratory under sterile conditions. The isolated bacteria were evaluated by polymerase chain reaction (PCR) to detect the genes encoding SEA and SEB. The results showed that 60 samples 20% were positive for S. aureus contamination. Among the strains of S. aureus, 21 samples 35% have sea gene and 9 samples 15% have seb gene. The presence of a 120 base pair fragment 120 bp related to the replication of the sea gene and the presence of a 478 base pair fragment 478 bp related to the replication of the seb gene indicated the presence of these genes in S. aureus. The high level of enterotoxin genes in S. aureus isolated from milk samples is a possible risk factor for consumer health. Therefore, it is necessary to improve the sanitary quality of milk in the study area.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | gene, sea, seb, enterotoxins, Staphylococcus aureus, Sistan |
| Subjects: | S Agriculture > SF Animal culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 15 Jan 2025 06:38 |
| Last Modified: | 15 Jan 2025 06:38 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3766 |
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