Lagzaei, Z. (2023) Investigating of the iron nanoparticles effects on clpP and HSP90 genes expressin in Moringa Oleifera plant. Masters thesis, university of zabol.
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Investigating of the zinc and iron nanoparticles effects on MO-CBP3 gene expression in Moringa oliefera medicinal plant.pdf Download (91kB) | Preview |
Abstract
Moringa oleifera is one of the plants that are widely distributed in tropical regions. It has great nutritional and medicinal benefits. It is rich in macronutrients and micronutrients and other bioactive compounds that are important for the normal functioning of the body and the prevention of some diseases. Moringa oleifera is the most studied among thirteen known species, proteins and important plant compounds with antioxidant activity have been found in its leaves. Studies on the clpP gene have shown that energy-dependent proteases play an important role in protein quality control by removing short-lived regulatory proteins and misfolded or damaged polypeptides. Also, heat shock protein 90 (HSP90) acts as molecular chaperones that support the correct folding of newly synthesized proteins as well as refolding and stabilizing the structures of previously folded proteins. Iron nanoparticles are widely used in medicine and industry today. Using nutrients as nanoparticles is a practical way to provide nutrients to plants. Iron is an essential micronutrient for plants. Therefore, the aim of this research is to investigate the expression of clpP and HSP90 genes in Moringa oleifera under the influence of iron nanoparticles. First, the seeds were cultivated, then the plants were affected by iron nanoparticle treatment in three repetitions with doses of 0, 20, 40, 60, and 0, 24, 48, 72 hours. Then Real Time PCR technique and SDHA reference gene were used to check their gene expression. And at the end, the analysis of the results was done using SAS 9. 1 statistical software, and analysis of variance and average comparison was done using Duncan's test. The results showed that the expression of ClpP gene at 72 hours and concentration of 20 and HSP90 at 48 hours and concentration of 20 had a significant increase compared to the control sample. Also, the highest amount of chlorophyll a and carotenoid was observed related to time 48 hours and concentration 40. According to the results of this study, the effect of iron nanoparticles and time increased the expression of ClpP and HSP90 and increased the amount of chlorophyll a and carotenoid
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Moringa oleifera, clpP gene, HSP90 gene, iron nanoparticles, Real Time PCR |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 06 Oct 2024 16:59 |
| Last Modified: | 06 Oct 2024 16:59 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3648 |
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