ali sofiyan, javad (2022) Evaluation of phytochemical, antimicrobial and anticancer properties of Neem (Azadirachta indica). Masters thesis, university of zabol.
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Investigation of phytochemical characteristics and bioactivity of Tanacetum parthenium rangeland during different phenological stages in Sarbisheh rangelands, South Khorasan province.pdf Download (260kB) | Preview |
Abstract
In order to investigate the phytochemical and anticancer properties of neem medicinal plant, a completely randomized experiment was conducted in the 1400 crop year. First, leaf samples (in the flowering phase of the plant, June) and neem fruit (in the fruiting phase, September) were collected from the Baloch nursery located in Bahuklat city. Samples in shaded conditions and normal dry room temperature and then ethanolic, methanolic, n-hexane and acetone extracts were prepared by cold maceration method with a ratio of 1:10. The content of total phenol was evaluated by Folin Ciocalto reagent method, total flavonoid by aluminum chloride colorimetric method and antioxidant activity by DPPH free radical inhibition method. Also, the oil of neem leaf and fruit samples was extracted by Soxhlet method and after calculating the percentage of oil, the fatty acid components in them were identified by GC-MS method. Finally, the anti-cancer effects of neem plant were investigated using MTT techniques, flow cytometry, real-time PCR and electron microscopy in colon cancer cells (HT-29). Antimicrobial effects of different neem fruit and leaf extracts on six strains of gram-negative and gram-positive pathogenic bacteria were evaluated by the minimum growth inhibitory concentration (MIC) and minimum lethal concentration (MBC) methods. The results showed that the most important components of neem leaf essential oil were caryophyllin oxide (%14/89), caryophyllin (%8/13), salvial oxide (%3/2) and kobaben (%3/55). Qualitative testing proved the presence of saponin, phenolic, tannin, cardiac glycosides and protein compounds in various extracts extracted from neem leaves and fruits. . The highest amount of total phenol (48.22 mg GA/g D.W.) in the acetone leaf extract, the highest amount of flavonoids in the leaf with values of 4.61 and 4.71 mgQ/g D.W., respectively. It was measured in the acetone extract of neem leaves and fruits. The highest antioxidant activity (%94/77) was observed from the ethanol extract of the leaf. Treatment of HT-29 cancer cells with concentrations of 5 and 10 μg/ml of neem leaf essential oil leads to induction Apoptosis was time-dependent in these cells. In the evaluation of antimicrobial properties, the lowest MIC and MBC (12.25 and 25 mg/ml) were observed from the ethanolic extract of neem fruit against Staphylococcus aureus and the methanolic extract of the fruit against Streptococcus pyogenes, respectively. In general, neem leaves and fruits were rich in phytochemical compounds that have good effects on human health, and further studies on its pharmacological properties are recommended.
| Item Type: | Thesis (Masters) |
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| Additional Information: | apoptosis, antioxidant, anticancer, total flavonoid, total phenol |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 27 Sep 2024 19:27 |
| Last Modified: | 27 Sep 2024 19:27 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3629 |
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