hajitabar, Mohammad (2023) Evaluation of the effect of HL-7 peptide extracted from Hemiscorpius Lepturus scorpion venom on the expression of caspase 8 gene in Hela cervical cancer cell line. Masters thesis, university of zabol.
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Evaluation of the effect of HL-7 peptide extracted from Hemiscorpius Lepturus scorpion venom on the expression of caspase 8 gene in Hela cervical cancer cell line.pdf Download (178kB) | Preview |
Abstract
Currently, cancer is one of the major causes of death in the world. One of the treatment methods is the use of bioactive peptides, which have fewer side effects. HL-7 peptide extracted from the scorpion Hemiscorpius lepturus is one of the bioactive peptides in the venom of this creature. In this study, the effect of HL-7 peptide at concentrations of 0, 45, 60 and 80 μM on the percentage of survival of normal cells (neutrophils) and at concentrations of 0, 45 and 60 μM on Hela cervical cancer cells after 12 , 24 and 48 hours using MTT test. Also, in order to measure the expression of genes at the mRNA level, Real Time PCR technique was used. The results showed that the IC50 of HL-7 peptide was 60 μM, which means that this concentration of HL-7 peptide decreased the viability of cancer cells by 50%. Therefore, 60 μM concentration of HL-7 peptide was used for further studies. Also, the results of Real Time PCR showed that the expression of caspase 8 gene at the mRNA level in Hela cancer cells treated with HL-7 peptide showed a significant decrease compared to untreated cells (p≤0.05). The results of the present study suggest that the HL-7 peptide extracted from H. lepturus scorpion venom has anticancer properties against Hela cells, which is promising for further investigations in in vivo and preclinical conditions. In the future, investigation of anticancer molecular mechanisms and relevant signaling pathways for HL-7 peptide will be studied to confirm the obtained results.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | gene expression, HL-7 peptide, Hemiscorpius lepturus, cancer, MTT |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 26 Sep 2023 06:24 |
| Last Modified: | 26 Sep 2023 06:24 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3383 |
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