Analysis of single nucleotide polymorphism (SNP) of PHLDA2 gene and its association with birth weight in Sistani Cattle

Jafarvand, N. (2015) Analysis of single nucleotide polymorphism (SNP) of PHLDA2 gene and its association with birth weight in Sistani Cattle. Masters thesis, University of Zabol.

Analysis of single nucleotide polymorphism (SNP) of PHLDA2.pdf

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Imprinted genes represent a small subset of mammalian genes that are mono-allelic ally expressed in a parent-of-origin manner. Imprinted genes are involved in fetal growth and placental development in the uterus. PHLDA2 is a member of imprinted gene family that has been described to maternally express in fetus and has limiting effects on fetal growth. To determine PHLDA2 gene polymorphism and its association with birth weight in Sistani cattle, the blood sample were randomly collected from male and female about 100 Sistani cattle located in research station Zahak. The DNA extraction was performed by using phenol chloroform procedures. Polymerase chain reaction was performed for amplification of 384 Bp of DNA segment located in exon 1of PHLDA2 gene using specific primer sets. For determining of possible mutations in target sequence the SSCP method, 8% polyacrylamide gel was used and for observation of fragments, polyacrylamide gel electrophoresis and silver staining were used. For investigating of the relationship between PHLDA2 gene polymorphism with birth weight and mean comparison, the least squares mean procedure (GLM) t- student test used by statistical software JMP. The results were showing that PHLDA2 gene has three band pattern (genotype) named as P1, P2 and P3, with frequencies of 0.45, 0.37 and 0.18, respectively. Statistical analysis has shown significant correlation between the genetic diversity in PHLDA2 gene and birth weight trait (P<0.05). Taken together these data suggest that the observed SNP within PHLDA2 gene could be used as molecular markers for the birth weight trait in Sistani cattle.

Item Type: Thesis (Masters)
Uncontrolled Keywords: PHLDA2, polymorphism, PCR-SSCP, Sistani cattle
Subjects: Q Science > QH Natural history > QH426 Genetics
S Agriculture > SF Animal culture
Depositing User: admin admin1 admin2
Date Deposited: 17 Apr 2016 09:10
Last Modified: 17 Apr 2016 09:10

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