Noura, M. (2022) Molecular identification of Brucella bacteria in clinical samples of Sistan region by multiplex PCR. Masters thesis, university of zabol.
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Abstract
Brucellosis or malt fever (Mediterranean fever) occurs due to the growth and multiplication of the gram-negative coccobacillus brucella in mammalian cells, this disease is known as a common disease between humans and animals, so the purpose of this research is to detect and identify the bacteria molecularly. Brucella using multiple PCR technique. To do this, first raw milk samples were collected from infected animals in Sistan region, then Brucella DNA was extracted. Also, genetic investigation was done by amplifying two genes, Omp31 and BLS, using multiplex PCR. The results of the molecular investigation of two genes, Omp31 and BLS, showed the amplification of two bands, 347 bp and 256 bp. Examining the specificity and sensitivity of Brucella bacteria with the Multiplex PCR method revealed 100% specificity and 0.39 ng⁄(μL ) sensitivity of genomic DNA. In this research, it was shown that the multiplex PCR molecular method has sufficient specificity and sensitivity to identify Brucella bacteria.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Brucella bacteria, Brucellosis, Malta fever, BLS and Omp31 genes, molecular identification, multiplex PCR |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 12 Sep 2023 06:53 |
| Last Modified: | 12 Sep 2023 06:53 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3360 |
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