Rahdar, S. (2022) The effect of biological elicitors on some morphological and physiological parameters of (Indigofera tinctoria L). Masters thesis, university of zabol.
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The effect of biological elicitors on some morphological and physiological parameters of (Indigofera tinctoria L).pdf Download (460kB) | Preview |
Abstract
Indigo plant (Indigofera tinctoria L.) is a plant belonging to the legume family (Fabaceae). This plant has therapeutic properties, such as: anti-inflammatory, blood fat reduction, nerve protector, anti-allergy, liver protector, anti-spasm and antioxidant. The purpose of this study was to investigate the effect of different levels of carrageenan (0, 100, 200 and 300 ppm) and chitosan (0, 200 and 400 ppm) on some morphological and physiological indicators of the Indigo plant. For this purpose, a factorial experiment was carried out based on a proprties random design in 3 replications in potted conditions in the research laboratory of the Faculty of Science University of Zabol in 1400. In addition, morphological traits including; Fresh weight, dry weight, stem length, leaf indices and some physiological parameters including; Photosynthetic pigments, total phenol (using Folin Ciocalto reagent method), total flavonoid (using aluminum chloride colorimetric method), antioxidant activity (using DPPH method) and activity of phenylalanine ammonialyase (PAL), polyphenol oxidase (PPO) and guaiacol enzymes. Peroxidase (GPX) was measured (by enzyme extract method). The results of variance analysis of the data showed different parameters including: weight, leaf indices, chlorophyll a and b, carotenoid, total phenol and antioxidant activity, PAL enzyme and PPO enzyme under the effect of spraying with carrageenan and chitosan elicitors in all concentrations increased significantly. Also, the interaction of these two treatments had a significant effect on the amount of dry weight; So that in the combined concentrations of 300 ppm carrageenan and 400 ppm chitosan, the highest weight (0.133 mg), leaf width (0.7 cm), carotenoid content (1.64 mg.g FW), total phenol content (109.43 mg.g FW) and antioxidant activity (88.83%) were observed and the maximum amount of chlorophyll a and b, dry weight, and PPO enzyme increased under the influence of the interaction of carrageenan and chitosan with a concentration of 200 ppm. The maximum amount of PAL enzyme (0.321 unit.mg protein) was seen in the combined concentrations of 100 ppm carrageenan and 200 ppm chitosan. No significant effect was observed in foliar application of carrageenan and chitosan alone and their interaction on the amount of flavonoid and the activity of guaiacol peroxidase (GPX) enzyme. In general, the effect of elicitors varies depending on the type and concentration of the elicitor and the type of plant species. Based on the results of this study, carrageenan and chitosan in high concentrations are suggested as suitable stimulants to increase growth and produce secondary metabolites.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | phenolic compounds, antioxidant activity, carrageenan, chitosan, indigo plant |
| Subjects: | Q Science > QP Physiology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 11 Sep 2023 07:37 |
| Last Modified: | 11 Sep 2023 07:37 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3345 |
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