Ebrahimnezhad, Z (2022) Evaluation of phytochemical compounds and antioxidant and antimicrobial properties of Stocksia brahuica. Masters thesis, university of zabol.
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Abstract
Stocksia brahuica, belongs to the family Sapindaceae, grows in Iran, Pakistan and Afghanistan as a thorny shrub with red inflated fruits. In this thesis, the phenolic and flavonoid contents, antioxidant properties and antimicrobial activities of the leaves, fruits and seeds of S. brahuica were investigated. For this purpose, a wild population of the plant was collected in the summer of 1400 from Goorband village in Zahedan. The total content of phenolic compounds in each organ was measured using Folin Ciocaltieu method and the results were expressed as milligram of gallic acid per gram of dry weight. The total flavonoid content was determined via aluminum chloride colorimetry and expressed as mg quercetin per gram of dry weight. The free radical scavenging method of 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to study the antioxidant properties of different organs. The antimicrobial properties of the hydroalcoholic extracts of leaves, fruits and seeds of the plant organs were invistigated against Gram-negative pathogens of Pseudomonas aeruginosa, Klebsiella pneumonia, Acinetobacter baumannii and Escherichia coli, Gram-positive bactera of Staphylococcus epidermis. and Streptococcus pyogenes as well as two fungal strains of Aspergillus fumigatus and Candida albicans. The results of this research showed that the total phenolic and flavonoid contents varies from 263.5 to 136.7 mg equivalent of gallic acid and 26.6 to 104.8 mg equivalent of quercetin per gram dry weight of the powders, respectively. The highest amounts of phenolic and flavonoid contents were found in leaves, fruits and seeds, respectively. Accordingly, the highest antioxidant activity was found in leaves (IC50=8.3) followed by fruits (IC50=25.2). and seeds (IC50=161.9). The antimicrobial results showed that the examined organs have little antimicrobial activity on the examined strains and only the seed extract was as effective as the antifungal drug, itraconazole, against Candida albicans strain. According to the obtained results, it can be concluded that the leaves, fruits, and seeds of S. brahuica do not show antimicrobial properties, but these organs, especially the leaves, contain high amounts of phenolic and flavonoid compounds and significant antioxidant activities. Therefore, the leaves of S. brahuica are designated as the organ with the highest potential for further studies for possible use in the food, pharmaceutical, and animal husbandry industries.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Total phenolic content, Flavonoids, Antioxidant capacity, Antimicrobial property, Stocksia brahuica. |
| Subjects: | Q Science > QP Physiology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 30 Jan 2023 09:04 |
| Last Modified: | 30 Jan 2023 09:04 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3269 |
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