Bahmani, Helia (2016) Hairy roots induction and secondary metabolite production in Satureja sahandica Using Agrobacterium rhizogenes. Masters thesis, University of Zabol.
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Hairy roots induction and secondary metabolite production in Satureja sahandica Using Agrobacterium rhizogenes.pdf Download (280kB) | Preview |
Abstract
Satureja sahandica, which is native to Iran, is a medicinal plant from Lamiaceae family and west of Iran is the only its habitat, which produces different types of plant secondary metabolites such as terpenes. Important monoterpenes such as thymol and carvacrol are the main components of essential oil of Satureja sahandica. Recently great efforts have been done for production of secondary metabolites by hairy roots induction in medicinal plants. Agrobacterium rhizogenesis transformation mediated has the high potential in the production of secondary metabolites in medicinal plants through induction of hairy roots. This study was performed in order to optimize the production of secondary metabolites in Sahandi savory through induction of hairy roots. Three different strains of Agrobacterium rhizogenesis (ATCC15834،A4 ،LBA9402) and four different explants (nodes, internodes, leaves and callus) were used as an exprimental design to optimize the induction of hairy root. The results showed that the ATCC15834, A4 and LBA9402 strains induced hairy roots in nodes and internodes and LBA 9402 only was effective in hairy root induction in one callus explant. More investigations revealed that nodal explants showed better respond to root induction, furthermore LBA9402 strain resulted the highest percentage of transformation and root number and length. Being transgenic of hairy roots was confirmed by PCR using gene-specific primers of rolB. Overall results showed that the bacterial strain type and kind of explants can have different effects on the induction of hairy roots. In order to increase biomass of hairy roots and more thymol production, effects of methyl jasmonate and ammonium nitrate treatments were assessed. Extraction from hairy roots and culture medium was done by using "N hexane" and "ethyl acetate" solvents, respectively. TLC and GC methods were used to measure the amount of thymol. Methyl jasmonate and ammonium nitrate compounds lead to a change in biomass production and the amount of thymol. In general, thymol measurement results showed that induced hairy roots synthetized more metabolites than the normal roots that this content was multiplied by applying methyl jasmonate and ammonium nitrate treatments.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Satureja sahandica, Agrobacterium rhizogenesis, Hairy root, thymol, Methyl jasmonate and ammonium nitrate |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 13 Nov 2022 09:28 |
| Last Modified: | 13 Nov 2022 09:28 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3234 |
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