Atashgah, M. R. (2022) Evaluation of aminoglycosides resistance pattern and aadB & aphA6 genes frequency In clinical strains of Acinetobacter baumannii in Zahedan. Masters thesis, University of Zabol.
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Evaluation of aminoglycosides resistance pattern and aadB & aphA6 genes frequency In clinical strains of Acinetobacter baumannii in Zahedan.pdf Download (29kB) | Preview |
Abstract
Introduction & Objective: Acinetobacter baumannii is one of the most important opportunistic pathogenic bacteria that causes serious nosocomial infections including ventilator-associated pneumonia, bacteremia, urinary tract infections, meningitis and surgical wound infections and is one of the most dangerous gram-negative bacteria. It infects approximately one million people worldwide each year, half of which are caused by multidrug-resistant strains of MDR. Antibiotic resistance to aminoglycosides in this bacterium is due to the acquisition, increased expression, or amplification of aminoglycoside-modifying enzyme genes and efflux pump 8. Aminoglycoside modifying enzymes include aminoglycoside acetyltransferase (AAC) aminoglycoside phosphotransferase (APH) aminoglycoside nucleotide transferase (ANT or AAD). Materials and Methods: In this cross-sectional study, 100 strains of Acinetobacter were collected from different clinical samples of patients referred to teaching hospitals in Zahedan. Discs were evaluated for gentamicin (10 μg), amikacin (30 μg), tobramycin (10 μg), and kanamycin (30 μg) discs. Then the bacterial genome was extracted and the presence of apha6 and aadB genes were identified using specific primers and PCR. Results: Acinetobacter baumannii isolates were resistant to kanamycin (86%), amikacin (82%), gentamicin (75%) and tobramycin (67%). Apha6 genes were detected in 56% and aadB genes in 5% of isolates. Conclusion: Due to the high resistance of Acinetobacter baumannii to most antibiotics, the results of this study show that resistance to aminoglycosides is increasing and these antibiotics are not a suitable option for the treatment of infections caused by Acinetobacter baumannii.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Acinetobacter baumannii, Aminoglycoside, apha6, aadB |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 08 Oct 2022 10:28 |
| Last Modified: | 08 Oct 2022 10:28 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3186 |
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