Barakzayi, M. (2015) Determination of TEM, CTX-M and SHV genes in Klebseilla pneumonia Producing extended spectrum beta-Lactamases using Multiplex PCR method. Masters thesis, University of Zabol.
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Determination of TEM, CTX-M and SHV genes in Klebsiella pneumonia.pdf Download (223kB) | Preview |
Abstract
Extended-spectrum beta-Lactamases of TEM, CTX-M and SHV type is considered as an Important mechanism resistant to cephalosporin in gram- negative pathogen and is widely increasing. Klebseilla pneumonia species are able to produce extended-spectrum beta- Lactamases (ESBLs). The aim of this study was to detect the prevalence of TEM, CTX-M and SHV genes in ESBLs producing Klebseilla pneumonia isolated form patients hospitalized in Zahedan hospitals by using PCR method. In this analytical- descriptive study antibacterial susceptibility patterns of 100 Klebseilla pneumonia tested to Cefotaxim, Ceftazidim, Ceftriaxon, Cefixime, Gentamicin, Tetracycline, Nalidixic acid, Imipenem, Co- Trimoxazole, Ciprofloxacin and Azithromycin using disk diffusion method. In addition, confirmatory test for detecting ESBLs phenotypes was performed using Cefotaxim-Clavulanic acid combination disk. The presence of TEM, CTX-M and SHV genes were assessed by using PCR. Confirmatory phenotypic test showed 59% of the isolates were ESBL positive. The prevalence of CTX-M and SHV genes in isolated Klebseilla pneumonia was 50% and 81% respectively. TEM genes were not found in any of the isolates. High frequency of CTX-M, TEM and SHV genes in ESBL producing isolates indicates that this enzyme plays an important role in resistance to beta lactam containing anti biotic.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Klebseilla pneumonia -Extended-spectrum beta-Lactamases, bla TEM, bla CTX-M, bla SHV genes |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 16 Apr 2016 08:06 |
| Last Modified: | 16 Apr 2016 08:06 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/318 |
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