The Prevalence of Salmonella Typhimurium and Salmonella Enteritidis by (PCR) molecular method in broiler poultry flocks of Khash city

CHALISH, MASOUD (2021) The Prevalence of Salmonella Typhimurium and Salmonella Enteritidis by (PCR) molecular method in broiler poultry flocks of Khash city. Masters thesis, University of Zabol.

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Abstract

Salmonella is one of the main health and economic problems in industrialized countries and salmonellosis is one of the most common diseases in the world. Poultry can play an important role in the spread of salmonellosis in humans.The aim of this study was to investigate the prevalence of Salmonella Serotypes Typhimurium and Enteritidis by molecular method (PCR) in broiler flocks in Khash city. 100chicken, 1 to 14 day old chickens were collected from 10 broiler farm in Khash city. Chickens collected under sterile conditions were transferred to the laboratory. Chicken livers and intestines were sterile sampled and cultured in nutrient agar culture. A total of 4 samples out of 100 cultured liver and intestine samples were positive. After biochemical analysis of isolated bacteria, DNA extraction was performed from the isolates. Then, polymerase chain reaction was performed for invA, sdf and STM genes. The results showed that the invA gene (Salmonella genus) with 100% frequency had the highest statistical percentage and then the sdf gene (Salmonella enteritidis) with 50% and STM gene (Salmonella typhimurium) with 0% had the lowest infection rate. In the present study, it was found that the prevalence of Salmonella enteritidis serotypes in broiler chickens 1-14 days old in Khash city is high.

Item Type: Thesis (Masters)
Uncontrolled Keywords: salmonella, enteritidis, typhimurium, PCR, kash, chicken
Subjects: Q Science > QR Microbiology
Depositing User: Mrs najmeh khajeh
Date Deposited: 03 Oct 2022 06:14
Last Modified: 03 Oct 2022 06:14
URI: http://eprints.uoz.ac.ir/id/eprint/3145

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