Namjoo, Moslem (2021) The effect of temperature changes on expression of HSP20 gene and detection of different Vibrio species in Pinctada radiata. Masters thesis, University of Zabol.
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The effect of temperature changes on expression of HSP20 gene and detection of different Vibrio species in Pinctada radiata.pdf Download (146kB) | Preview |
Abstract
Edible oysters are one of the most important marine farming organisms that their consumption in raw or semi-cooked form by humans causes disease, one of the causes of which is the presence of pathogenic Vibrio bacteria in oysters. Physiological parameters of bivalve molluscs such as reproduction, metabolism, safety parameters, etc. in mollusks are very sensitive to temperature changes. The HSP1 gene consists of several families that are affected when aquatic organisms are exposed to stressful conditions (salinity, temperature, etc.). The aim of this study was to investigate the expression of HSP20, HSP70 and HSP90 genes in the gill tissue of oyster shells under temperature treatments of 22, 25, 27 and 29 ° C and the development of Vibrio bacteria. Gene expression was studied by real time PCR technique and GADPH control gene were used in data normalization. Data analysis was performed using SAS software to investigate the presence or absence of significant differences between means (at a significant level of 5%). The results of the analysis showed that the expression of HSP20, HSP70 and HSP90 genes at 25 °C was significantly increased compared to the control and the highest expression was observed in ocular gill tissue at 25 °C. Also for HSP20 and HSP70 genes the lowest expression was observed at 29 °C, while for HSP90 gene the lowest expression was observed at 27 ° C. According to this study, the incidence of Vibrio bacteria in temperature treatments of 22, 25, 27 and 29 °C in oysters was investigated using 16S rRNA gene amplification. 16S rRNA gene sequencing was performed and sequence alignment analysis was performed using sequence BLAST in NCBI database. The alignment results of 16S rRNA gene sequence showed 100, 100, 99.92, 99.99 similarity of PM1, PM3, PM5, PM10 and PM19 strains with Vibrio anguillarum, Vibrio parahaemolyticus, Vibrio harveyi NBRC 15634 ،Vibrio harveyi NCIMB1280 and Vibrio alginolyticus. Isolation and counting of Vibrio bacteria was performed in each temperature treatment. According to the results, with decreasing temperature from 29 to 22 ° C, the frequency of isolates belonging to V. parahaemolyticus decreased from 30% to 12% and the frequency of isolates belonging to V. alginolyticus decreased from 25% to 10%. The prevalence of other non-pathogenic species of Vibrio increased from 15% at 29 ° C to 40% at 22 ° C. Therefore, isolates of V. harveyi had the highest frequency at 22 ° C and the lowest frequency at 29 ° C. According to the results of this study, temperature was effective in the expression of HSP20, HSP70, HSP90 and Vibrio bacteria.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Oyster, Temperature, HSP20 genes, HSP70, HSP90, Vibrio bacteria, Real Time PCR |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 03 Oct 2022 05:49 |
| Last Modified: | 03 Oct 2022 05:49 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3140 |
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