ARAB, T. (2014) Determination of presence of CTX-M genes in of Klebseilla Pneumonia strains isolated from hospitals in Zabol. Masters thesis, University of Zabol.
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Abstract
Resistance to antibiotic is important problem to treat of patient. There has a dramatic increase in the number of organisms that produce extended-spectrum Betalactamases. CTX-M-type enzymes constitute a distinct lineage of molecular class a beta-Lactamases, and are a rapidly growing group. These beta-Lactamases consist of four groups. This class of beta-Lactamases has been recognized worldwide as an important mechanism of resistance to Oxyiminocephalosprins used by gram-negative pathogens. In this analytical-descriptive study, antibacterial susceptibility patterns of 70 Kelbsiella to Cefotaxim, Ceftazidim and Ceftriaxon tested using disk diffusion method. In addition٫ confirmatory tests for detecting ESBLs phenotypes were performed using Ceftazidim-Clavulanic acid combination disk. The PCR assay was used for detection of CTX-M genes. From 70 clinical isolates 70 isolates were positive for ESBL in initial screening tests and from them 66 isolates were positive in phenotypic confirmatory tests. 66 isolates potentially producing extended spectrum- beta-Lactamases were used for the presence of CTX-M genes by molecular assay. From 70 clinical 46.62% were positive for BLA group I genes, 5.38 % was positive for BLA group II genes.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Klebseilla pneumonia, extended-spectrum beta-Lactamases, CTX-M genes, PCR |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 16 Apr 2016 07:36 |
| Last Modified: | 16 Apr 2016 07:36 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/312 |
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