Zarei, H. (2021) Study of evolutionary and phylogenetic relationships and identification candidate miRNAs in Iranian Allium species. Doctoral thesis, University of Zabol.
|
Text
Study of evolutionary and phylogenetic relationships and identification candidate miRNAs in Iranian Allium species.pdf Download (138kB) | Preview |
Abstract
Allium L. is one of the largest genera of the Amaryllidaceae family, with more than 920 species including many economically important species used as vegetables, spices, medicines, or ornamental plants. Currently, DNA barcoding tools are being successfully used for the molecular taxonomy of Allium. MicroRNAs (miRNAs) are a class of endogenous small regulatory RNAs consisting of about 18–22 nucleotides derived from their precursor sequences. In this study to investigate phylogenetic relationships using matk chloroplast marker we used a total of 46 species of Allium. Sequencing results and analysis of the data and generated phylogenetic tree with several method such as Neighbor-joining shown that, The NJ dendrogram generated three distinct clades: the first contained Allium austroiranicum and A. ampeloprasum; the second contained A. iranshahrii, A. bisotunense, A. cf assadi and A. rubellum. the third contained and other species. according to the rooting of the tree, the first two species are older than the other species. In this study, EST-based methods were used to identify miRNA in Allium. A total of 45,510 reported Allium EST sequences were received and after BLASTn and BLASTx with all miRbase sequences and deleting protein coding sequences, finally miR169 and miR171 sequences with 9 EST sequence of Allium had homology and And were selected as miRNA candidates. After designing specific primers and performing RT PCR and Real-Time PCR and band and The amplification curve observation, also indicated the presence of these miRNA sequences in Allium.
| Item Type: | Thesis (Doctoral) |
|---|---|
| Uncontrolled Keywords: | Allium, matK, phylogeny, miRNA, EST |
| Subjects: | Q Science > QK Botany |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 02 Oct 2022 06:57 |
| Last Modified: | 02 Oct 2022 06:57 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3113 |
Actions (login required)
 |
View Item |
