Sobhani moghadam, Sudabe (2021) Evaluation of Atg8 gene expression changes in transgenic Aβ and TAU models of Drosophila. Masters thesis, University of Zabol.
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evaluation of Atg8 gene expression changes in transgenic Aβ and TAU models of Drosophila.pdf Download (90kB) | Preview |
Abstract
Autophagy, meaning self-eating, is a cellular process in which the cytoplasmic content (old proteins and damaged organs) are enclosed within bilayer vesicles and, after joining the lysosome, break down to produce energy. Autophagy is often used as a mechanism to maintain cell survival, but excessive progression of autophagy can lead to cell death. One of the genes involved in autophagy is Atg8. The Atg8 gene is very important in macroophagy. Macroatophagia is one of the three distinct types of autophagy, characterized by the formation of vesicles enclosed in a double membrane and separating parts of the cytosol called autophagosomes. In Alzheimer's disease, tao protein tangles, along with amyloid beta extracellular plaques, are the second most damaging factor to nerve cells. One of the pathways in which tao and amyloid beta can be pathogenic can be disrupted by inhibiting the autophagy pathway. Drosophila melanogaster as a suitable laboratory model due to having a wide range of advanced genetic techniques for genetic instructions as an efficient and cost-effective system can help researchers to understand this issue. In the present study, we determined the changes in Atg8 gene expression by realtime PCR technique in the transgenic amyloid beta and tao models of vinegar fly. In this study, UAS / TAU, UAS / AB42 elav / Gal4 and Repo / Gal4 Gmr / Gal4 lines were used. Thus, virgin Gal4 lines were taken and then mated with UAS male flies and first generation offspring were examined. The accuracy of the model was confirmed, after which RNA extraction and CDNA synthesis were performed and Atg8 gene expression was evaluated by Real time PCR. The results showed that the expression of wild and mutant version of human TAU in the central nervous system of Drosophila increased the expression of Atg8 gene. Also, the expression of AB42 in the central nervous system of Drosophila increased the expression of Atg8 gene.
| Item Type: | Thesis (Masters) |
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| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 24 Sep 2022 06:08 |
| Last Modified: | 24 Sep 2022 06:08 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/3027 |
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