Rafat, S. (2009) Morphological and Cytogenetical Studies of Double haploid Barley Lines. Masters thesis, University of Zabol.
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Morphological and Cytogenetical Studies of Double haploid Barley Lines.pdf Download (162kB) | Preview |
Abstract
In order to study morphological variations of 81 double haploid lines of barley and determination of the relationships between several traits with each other, the morphological experiments was arranged as randomized complete block design with 3 replications. Cytogenetical surveys were performed for study of cytogenetical replication in 21 genotypes. The experimental design was a complete randomized factorial, with 3 replicates and with tow factors of genotype and chromosome that had 21 and 7 level, respectively. karyotypical researches showed that all the genotypes had different numbers of satellite chromosomes except to genotypes (54,62). The highest mean of chromatin length was related to genotype number 80 (10.247 μm) and the lowest of it was related to genotype number 73 (3.945 μm). Types of choromosomes were determined by Levan et al method. In this research, evolutional situation and karyotypical symmetry were evolutes based on some parameters such as percent of karyotype general form, percent of chromosome form symmetry index, coefficient of variation, difference between relative length range and dispersion index. Assigning of karyotypical symmetry was carried out by Stebbins method and genotypes were divided to 4 symmetrical classes. The calculated dispersion index also emphasized. These results the highest amount off this index was related to genotype number 48 (2.2) and the lowest of it was related to genotype number 54 (0.97). The karyotypes were classified by Romero- Zarco method. The highest for A1 = 0.98 was genotype number 48. Analysis of variance results for morphological traits indicated that there were significant differences among the genotypes for most of the traits. In following studies, correlation between genotypes was determined for morphological traits and chromosomal and karyotypical features. Cluster analysis placed the genotypes in 8 groups for all morphological traits and 4 groups for chromosomal traits. Principle components analysis was explained ٪96.7 of total variance, with the extraction of 7 components for morphological characters and it was explained ٪99.9 of total variance, with the extraction of 3 components for chromosomal parameters. The results of step wise regression showed that 10 traits explained ٪89.8 of total variation of grain yield. In path analysis, the traits of number of seed per panicle, number of panicle per plant and 1000 seed weight had the highest direct effects on yield. For morphological traits, factor analysis extracted 7 invisible factors, which justified more than ٪75 of total variance. And for karyotypical traits, 3 factors justified more than ٪99 of total variance. Based on cluster analysis on morphological traits, they classified in 4 groups in, between these traits the seed per panicle and panicle length traits had the nearest interval to yield. Finally, the conducted researches revealed that in order to obtain high yield in barley, plants have to be selected with most number of seed per panicle, 1000 seed weight and longer panicle length. In accordance with results of morphological and chromosomal classifications, the parents must be selected in cross programs that have more yield and they are located in one group in chromosomal traits classifications.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | double haploid barley, morphological variations, cytogenetically variations, karyotype, chromosome and multivariate techniques |
| Subjects: | Q Science > QK Botany |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 13 Sep 2022 07:58 |
| Last Modified: | 13 Sep 2022 07:58 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2950 |
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