Jami, A. (2019) Effect of lactobacillus bacterial additive and fibrolytic enzyme on in situ ruminal degradation and in vitro gas production of sorghum forage silage. Masters thesis, University of Zabol.
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Effect of lactobacillus bacterial additive and fibrolytic enzyme on in situ ruminal degradation and in vitro gas production of sorghum forage silage.pdf Download (142kB) | Preview |
Abstract
In order to investigate the effect of lactobacillus bacterial additive and fibrolytic enzyme on the in situ ruminal degradations and in vitro gas production of sorghum forage silage, a factorial experiment based on completely randomized design with three replications was carried out at the animal nutrition laboratory, of Zabol university. Treatments were lactobacillus at 3 levels (0 and 1.5×108 and 3×108 g/kg dry matter) and fibrolytic enzyme at 3 levels (0 and 0.5 and 1 g / kg dry matter) in total with 9 treatments including treatment 1: Bacterial control with enzyme control (no additive), Treatment 2: (Bacterial control with 0.5 g enzyme), Treatment 3: (Bacterial control with 1 g enzyme), Treatment 4: (1.5×108 g bacteria with enzyme control), treatment 5: (1.5×108 g bacteria with 0.5 g enzyme), treatment 6: (1×108 g bacteria with 0.5 g enzyme), treatment 7: (3×108 g bacteria with enzyme control), treatment 8:(3×108 g bacteria with 0.5 g enzyme), treatment 9: (3×108 g bacteria with 1 g enzyme per kg dry matter) silage making and evaluated. The results showed that the highest percentage of dry matter (96.93%), sorghum silage organic matter (93.52%), crude protein percentage (9.68%), sorghum silage crude fat percentage (5.42%) and highest water-soluble carbohydrate content (9.36%) was related to the treatment 9. Also the highest percentage of ash (11.19%), percentage of insoluble fiber in acid detergent (37.41%), percentage of insoluble fiber in neutral detergent (48.88%), pH (4.79), and highest ammonia nitrogen content (14.93 mg / dl) was related to the control lactobacillus and fibrolytic enzyme treatments. The comparison of the interaction effects showed that the highest quality index (33.87) was related to the application of treatment 5. All silages were evaluated in a good and excellent range in appearance. Color index, odor, texture, and plant tissue of sorghum silage in the application of treatment 5 had the highest score (18.42) and evaluation of this treatment was very good. The highest percentage of gas production was observed in the treatment application of treatment 9 at 48, 72, and 96 hours. The highest rate of rapid degradable (a) and slow degradable (b) were related to the application of treatment 9. The combination of the two additives improved the potentially degradable fraction (a+b), which was highest with 81.90% in the treatment 9 that there were statistically significant differences between treatments. The combination of two additives had a significant degradable rate constant (c) and the highest rate of degradation was observed in the control lactobacillus and fibrolytic enzyme treatments. The highest volume of gas produced at 12, 24, 48, 72, and 96 hours were related to the treatment of treatment 9 and the highest volume of gas produced at 3 and 6 hours was related to the application of treatment 9 and at all incubation times the lowest amount of produced gas was observed in the control treatment. Based on these results, it can be concluded that the combination of lactobacillus and fibrolytic enzyme improves the nutritional value of sorghum silage.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Enzyme, Lactobacillus, Sorghum forage, Nylon bag, Silage |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 13 Sep 2022 04:20 |
| Last Modified: | 13 Sep 2022 04:20 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2932 |
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