Bagheri, R. (2020) Investigating the salinity and gold nanoparticle effects on Quercetin 3-o-metheyltransferase gene expression in Chenopodium quinoa. Masters thesis, University of Zabol.
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Investigating the salinity and gold nanoparticle effects on Quercetin 3-o-metheyltransferase gene expression in Chenopodium quinoa.pdf Download (275kB) | Preview |
Abstract
Quercetin 3-O-methyltransferas gene is an important gene because of its medicinal charcteristics including anti-diabetic, anti-cancer and antialzheimer disease. The aim of this research is to study the effects of salinity stress and gold nanoparticle on expression of this gene in Chenopodium quinoa. First, quinoa seeds in pots were randomly planted in four concentrations of salinity and gold nanoparticles, each with three repeats. the completetion of the fourth leaf, salinity treatment was performed at 4 After concentrations of 0, 200, 400 and 600mM and nanoparticles at 4 concentrations of 0, 45, 35 and 25ppm, later RNA extraction from the leaves of plant was done at 3 different time periods of 24, 48 and 72 hours after the treatments. For normalization of the data was used 18s rRNA as control gene and gene expression was evaluated by real-time PCR. Data analysis was performed by Graphed Prism 8 and confirmatory test of correction and One way-Anova by p value<0.05. The real-time results showed that by decreasing the gold nanoparticle up to 35ppm gene expression also upregulated and its highest level occurs at 72 hours, on the other hand at 300ppm concentration of gold nanoparticle at 72 hours the aformentioned gene was downregulated. As regard to salinity stress all 3 concentration caused the upregulation of the gene at 24 hours and the decrease continued till 400ppm. Overall in the process of aging the expression of quercetin 3-O-methyltransferas gene enhanced under treatment of gold nanoparticles, while; gene expression under salinity stress decreased by age.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | quercetin 3-O-methyltransferas gene, quinoa, gene expression, salinity stress, gold nanoparticle, Real time PCR |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 20 Oct 2021 05:32 |
| Last Modified: | 20 Oct 2021 05:32 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2826 |
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