Saboori-Robat, Elham (2019) Transformation of glyphosate herbicide resistance gene into soybean using Agrobacterium. Doctoral thesis, university of zabol.
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Abstract
Soybean is considered as one of the best sources of protein for the nutrition of humans and mammals, and also is cultivated as an economic source of both vegetable oil and protein. Soybean like other Leguminosae, contains low levels of Samino acids (methionine and cysteine). The purpose of this study is transferring 11 kDa delta‐zein and EPSPS genes to improve the methionine content and induce resistance to glyphosate herbicide. The presence of 11 kDa delta‐zein and EPSPS genes in T0 and T1 transgenic plants was confirmed by PCR. Shikimic acid and Chlorophyll content analysis also revealed that these two indexes in transgenic lines compared to wild type were significantly decrease and increase, respectively after glyphosate application. Also, in order to better understand the pathway of cysteine biosynthesis in legumes, we analyzed the sulfur amino acids and expression levels of genes involved in the biosynthesis of cysteine in developing seeds of the model plant (common bean). Metabolite profiling during seed development in total Smethylcysteine accumulation showed that γ-glutamyl-S-methylcysteine accounts for most of the total S-methylcysteine in mature seed. Correlation between the expression of BSAS4;1 gene and the S-methylCys concentration in early developmental stage of seed indicated that BSAS4,1 can be considered as a candidate gene for the synthesis of S-methylcysteine. Also, the expression of hGS may regulate the accumulation of γ-glutamyl-S-methylcysteine, through the provision of homoglutathione and S-methylhomoglutathione.
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | glyphosate, methionine S-methylcysteine, γ-Glu-S-metCys, BSAS4;1. |
| Subjects: | Q Science > QK Botany |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 30 Jun 2020 06:52 |
| Last Modified: | 30 Jun 2020 06:52 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2721 |
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