Morphological and physiological study of three halophyte species Salicornia persica, S.perspolitana and S.biglovii

Tayebi, Behrooz (2019) Morphological and physiological study of three halophyte species Salicornia persica, S.perspolitana and S.biglovii. Doctoral thesis, university of zabol.

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In order to compare the morphological and physiological responses of three salicornia species to NaCl concentrations, a greenhouse experiment was carried out. The factors was consisted of five levels of NaCl concentrations (5, 100, 200, 400 and 600 mML-1) and three Salicornia species (Salicornia persica ، Salicornia perspolitana and Salicornia bigelovii ). The results showed that, simple effects of salinity and species on proline, glycinebethein, carbohydrates, chlorophyll a, chlorophyll b, relative water content, osmotic potential, dry and fresh weight and antioxidant enzymes CAT,APX and GPX were significant. Also, interaction between salinity and species was significant on proline, glycine betaine, carbohydrates, chlorophyll a, chlorophyll b, osmotic potential, dry and fresh weight and CAT,APX and GPX. Carbohydrates concentration increased to 70-80% up to 400 mM and then decreased from 5% to 13% in 600 mM. Increasing in salt concentration up to 600 mM, increased proline and glycine betaine concentrations. Osmotic potential was also 3 to 4 times higher with increasing salinity levels. The chlorophyll a and chlorophyll b content decreased up to 600 mM. Also, at 200 mM NaCl in all of three species, wet and dry weights were significantly increased 2 to 3 times as control, and in 600 mM, Decreased 60% to 65%. Also, the activity of antioxidant enzymes APX, GPX and PAL increased up to 600 mM,compared to the control by 1.5, 6 and 5.5 times respectively. CAT enzyme activity increased up to 200 mM compared to the control by 1.7 times and then decreased by 42%. In summary, the results showed that, increasing in salt concentrations in the root environment, all three species survived in high salt concentrations with proline and glycine-betaine accumulation and through reduction of osmotic potential that Increased the activity of APX, GPX and PAL enzymes while Eliminate ROS radicals. S.bigelovii had the highest Na+ accumulation in 600 mM of NaCl and less accumulation of K+, Ca2+ and Mg2+. On the other hand, S.perspolitana In 600 mM NaCl concentration had the highest accumulation of potassium, calcium and magnesium, and in terms of antioxidant enzymes activity was higher than other species in salinity levels. The results showed that S.perspolitana is the species with higher salinity resistance among the species due to higher photosynthetic capacity retention through high hotosynthetic pigments under severe salinity stress and also maintaining higher potassium to sodium ratio. Also, given the higher antioxidant capacity of this species, especially the higher CAT enzyme levels in severe salinity stress, it can be concluded that the resistance of the latter species to salinity had significantly correlated with the increase in antioxidant enzymes.

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: Carbohydrates, Chlorophyll, Osmotic potential, Proline, Relative water content
Subjects: S Agriculture > SB Plant culture
Depositing User: Mrs najmeh khajeh
Date Deposited: 18 Feb 2020 06:58
Last Modified: 18 Feb 2020 06:58

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