Ayati, Simin Sadat (2019) Identification of Enterotoxin, Toxic shock syndrome and Exfoliative toxin gene of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus isolates collected from clinical samples. Masters thesis, university of zabol.
|
Text
Identification of Enterotoxin, Toxic shock syndrome and Exfoliative toxin.pdf Download (211kB) | Preview |
Abstract
Staphylococcus aureus produces a wide variety of exotoxins that contribute to its ability to cause disease in hosts. The pyrogenic toxin superantigens (PTSAgs) are a group of exotoxins secreted by some strains of S. aureus. The family of PTSAgs presently includes TSST-1 and most of the staphylococcal enterotoxins. Identification of these factors is essential in the recognition of Pathogenic bacteria. The main objective of this study was to identify Enterotoxins A ،B ،C، D ،E Toxic shock syndrome (TSST-1) and Exfoliative toxin (A/B) genes of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus isolates collected from clinical samples by PCR method. The present study was conducted on 60 Staphylococcus aureus isolates. Methicillin-resistant and susceptible isolates were detected by oxacillin screening agar test as per CLSI guidelines. The bacterial genomic DNA was extracted by boilling method. Prevalence of genes encoding Toxic shock syndrome (tst), Exfoliative toxin (ETA/ETB), and Enterotoxin (seA, seB, seC, seD, seE) were detected by PCR and specific primers. Of total 60 S.aureus isolates 32 and 28 isolates were methicillin-resistant and sensitive, respectively. Frequency of enterotoxin, exfoliative and toxic shock syndrom genes in resistant isolates were %50, %21/87, %3/12, respectively. In sensitive isolates the prevalenece of above genes were %10/71, %7/14, and % 0, respectively. The results indicated that the prevalence of methicillin resistant isolates and the strains carrying causative gene for Enterotoxin and Exfoliative, is high in Sistan regain. Also, circulation of these isolates can lead to much more severe effects in individual patients with weak immune system.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Staphylococcus aureus, Enterotoxin, Exfoliative, Toxic shock Syndrom |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 18 Feb 2020 06:48 |
| Last Modified: | 18 Feb 2020 06:48 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2686 |
Actions (login required)
 |
View Item |
