Abouei, M (2016) Identification of self-compatibility and self incompatibility in almond genotype (Prunus dulcis Mill.) by PCR-based markers. Masters thesis, university of zabol.
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Abstract
One of the problems of almond production is the issue of conveniet pollination assurance, due to self incompatibility. Self-incompatibility causes the extreme alleviation in formation of fruit and as a result, it creats some problems in managment of almond gardens. Most varieties of almonds have gametophytic self incompatibility, that is controlled by a genetic place with several type of alleles. The deterent insemination factor in this system is the deterrance growth pollen tube in style. In this study, in order to identify S allele in different genotypes were used from specific primer pairs such as AS1II-AmyC5R, ConF-ConR and Cebador2-Cebador8 in PCR as a control factor. Primers of AS1II-AmyC5R and Cebador2-Cebador8 constitued bands of 1200pb for Sf allele. Using primers S alleles S1, S2, S3, S10, S11, S23 and S31 in samples of selfincompatibility were determined by use of primer pairs ConF-ConR. Known alleles S1, S2, S3/Sf, S5/10, S11, S23 and S13 were identified by use of primer pairs AS1II-AmyC5R. The other bands that are derived from the production of PCR are related to self incompatibility S allele, which maybe related to new incompatibility alleles.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Prunus dulcis, Self-incompatibility, PCR, Molecular Markers |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 10 Dec 2019 07:00 |
| Last Modified: | 10 Dec 2019 07:00 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2623 |
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