Alayee, Gh (2019) Frequency of Iron transmitter genes in E. coli isolation in poultry or APEC. Masters thesis, university of zabol.
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Abstract
Although Escherichia coli is present in the natural intestinal flora and other host mucosal surfaces and in the bird environment, only some of these species, which have specific virulence characteristics, are known as pathogenic Escherichia coli. They cause disease. APEC, or avian pathogenic Escherichia coli, is more likely to be associated with intestinal infections, respiratory tract infections or systemic infections, leading to a variety of diseases that are responsible for the severe economic damage to the poultry industry. Iron is an essential factor for many cellular biochemical activities, such as the electron transport chain. Aerobactins are the iron-absorbing bacterial agent found in Escherichia coli. The main objective of this study was to evaluate the frequency of feoB, ireA, fyuA, prrA, modD, fepC, hlyB and hlyC pathogenic iron transmitter genes as effective genes in iron uptake process in Escherichia coli by Multiplex-PCR technique. The fyuA gene, with 60% of observations, was the most abundant among the 100 isolates. Then feoB genes with 53%, fepC with 53%, hlyC with 39%, hlyB with 36%, modD with 21%, ireA with 11% and prrA with 10% were observed. Therefore, it was found that the fyuA gene with 60% had the highest frequency and the prrA gene with 10% had the lowest gene frequency among APEC isolates in Sistan region. By identifying APEC pathogenic genes in chronic infections and at the expense of costly treatment each year, vaccine design against this disease can be suggested.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Pathogenic Escherichia coli, Iron transmitter genes, poultry |
| Subjects: | S Agriculture > SF Animal culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 26 Nov 2019 06:06 |
| Last Modified: | 26 Nov 2019 06:06 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2601 |
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