Ghafari, Maryam (2019) The study of broiler infection to Campylobacter jejuni and Campylobacter coli in the final breeding ages by multiplex PCR in Zabol. Doctoral thesis, university of zabol.
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Abstract
Background and Objective: Campylobacter is a common type of bacteria in humans and poultry, which generally accounts for various diseases in humans and commonly, poultry have a significant role in human campylobacteriosis. The aim of this study was to evaluate the prevalence of Campylobacter in the poultry cecum which sampled from broiler chickens. Materials and Methods: This randomized clinical trial was conducted in the city of Zabol, Iran. A total of 100 samples of chicken cecums were randomly collected from the 20 aviculture in Zabol. After sampling in aseptic environment and on ice, samples were transferred to the laboratory and evaluated by multiplex PCR method. By which, PCR products were electrophoresed on a 1.5% agarose gel and 589 bp for Jejuni type as well as 465 bp for Coli type, were have been considered. Results: According to the results, 16 of the samples were found to be infected by C. Jejuni and 29 of them were infected by C. Coli. The infection prevalence showed a significant difference between these two types of Campylobacter (P value= 0.011). But, on the other hand, there were no significant difference had founded between fall and winter in both C. Jejuni (P = 0.538) as well as C. Coli (P = 0.899). the findings were same as the former results for over and under 30-day chikens for C jejuni, but the prevalence of C. Coli between these two periods were founded to be significant (P = 0.001). Conclusion: According to this study the broiler poultry in Zabol had a relatively high prevalence of C. jejuni and C. coli, Therefore, due to the infective effect of eating infected chickens, in human, it seems that knowing the infection risk factors and its related control mechanism is vital and necessary.
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | Campylobacter jejuni, Campylobacter coli, broiler chiken |
| Subjects: | S Agriculture > SF Animal culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 17 Nov 2019 05:37 |
| Last Modified: | 17 Nov 2019 05:37 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2587 |
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