Golshani, Farshid (2019) Evolutionary and phylogenetic relationships of wild species and cultivated Iranian saffron based on DNA barcoding. Doctoral thesis, university of zabol.
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Abstract
DNA barcoding method was applied to identify and study the phylogenic relationships existing between 15 species of saffron collected in Iran including 4 crop and 11 wild species. DNA extraction was done by modified Delorapurta method. A total of 60 sequences were obtained and registered in NCBI database. In particular, 25 of these sequences (rbcL, n=11; matK, n=4; trnH-psbA, n=5; ITS, n=5) were not already present in the scientific library. Nucleotide polymoprhic sites were counted for each barcode gene (rbcL, n=16; matK, n=15; trnH-psbA, n=46; ITS, n=71). Each sample could be distinguished from the others in the phylogenic trees developed based on the data obtained by single barcodes (rbcL ،matK and trnH-psbA ) genes to genus level. sequence rbcL has low resolution at the species level, it can contribute to interspecific studies in combination with matK sequence. The disadvantages of trnHpsbA sequence, are presence of repetitive positions and inappropriate sequence alignment due to its variable length among samples. matK phylogenetic tree seemed to enjoy greater discrimination power compared to rbcL and trnH-psbA data. It can therefore be used as a complementary barcode with other barcodes. In general, ITS sequence, indicating high resolution at the genus and species level, appeared as the best barcode sequence of the present study. In addition, a phylogenic tree based only on plastid information (trnH-psbA + rbcL + matK) and another created on the data resulting from both nuclear and plastid genomes (trnH-psbA + rbcL + matK + ITS) were also generated. In general, using combination barcode sequences (trnHpsbA+ rbcL+matK+ITS), indicating high resolution at both genus and species levels and were used to reconstruct phylogenetic events in Crocus genus. Phylogenic analysis demonstrated the genetic relationship between crop saffron and wild Crocus species. As a result of this study based on 15 species of Crocus family, wild species C. sp. Eslamabad and C. cancellatus were close to Iranian saffron. Phylogenetic analysis based on 41 species of Crocus family through ITS barcode revealed the genetic relationship between Crocus sativus and Crocus wild species, correctly and with high resolution. As a result, the closest relatives of Iranian saffron are C. cartwrightianus and C. oreocreticus and C. cartwrightianus is the ancestor of C. sativus. Our investigation also indicated that the different ecotypes of C.sativus may have evolved through independent events probably due to geographic and environmental pressures
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | phylogeny; DNA barcoding; Crocus; rbcL; matK; trnH-psbA; ITS |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 11 Nov 2019 05:54 |
| Last Modified: | 11 Nov 2019 05:54 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2569 |
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