Ganjali, A. (2014) Frequency determination of Metallo--lactamase genes blaIMP-1, blaVIM-1 and blaSPM-1 in Pseudomonas aeruginosa strains isolated from hospitalized patients in Zabol city. Masters thesis, University of Zabol.
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Abstract
Pseudomonas aeruginosa is a Gram-negative bacterium that plays a major role in development of opportunistic and severe infections in hospitalized patients. Occurrence of enzymes and capable of inactivating all beta-lactams including carbapenems is new problem in treatment of patients. The objective of this study is to investigate the prevalence of Metallo-Beta-Lactamases (MBL) enzymes blaIMP-1, blaVIM-1 and blaSPM-1 in P. aeruginosa strains isolated from patients attending teaching hospitals in Zabol, Iran by PCR method. Conventional Biochemical Tests identify one hundred of isolated P. aeruginosa. Kirby-Bauer method performs antibiotic susceptibility test. Double Disc Synergic Test (DDST) method is used for phenotypic detection of MBL and blaIMP-1, blaVIM-1 and blaSPM-1 genes, which are determined by PCR method using specific primers. Out of 100 isolates, 11%, 7%, 15%, 98%, 4% and 7% are resistant for imipenem, ciprofloxacin, ceftazidime, cefixime, tobramycin and piperacillin, respectively. MBL enzyme is detected in 5% of isolates. All of phenotypically MBL isolates had blaVIM-1 gene. 77% and 12% of isolates had blaVIM-1 and blaIMP-1 respectively and 10% of isolates had blaVIM-1 and blaIMP-1 simultaneously. None of the isolates had blaSPM-1. The results of this study show that the prevalence of MBL enzymes in patients attending teaching hospitals in Zabol is very low, antibiotic resistance for cefixime was high, and it is necessary to determine susceptibility test before treatment
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Metallo-Beta-Lactamases (MBL) Enzymes, , Pseudomonas aeruginosa, Antibiotic resistance |
| Subjects: | Q Science > QH Natural history > QH426 Genetics |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 05 Apr 2016 09:09 |
| Last Modified: | 05 Apr 2016 09:09 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/255 |
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