Morphological and Molcular analysis of Nematoda (Anizakidae) in, epinephelus of Oman Sea

Afzali, Mohammad (2019) Morphological and Molcular analysis of Nematoda (Anizakidae) in, epinephelus of Oman Sea. Masters thesis, University of Zabol.

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As the population grows, the need for more fish is needed. Many species of fish are a potential source of common parasites between humans and fish. One of the important diseases between human and fish is Anisakiasis . The parasitic agent of this disease is the larval stage of the Anisakid family nematodes, including pseudoterranova and Anisakis. In this study, Epinephelus fish, one of the commercial fishes of the Oman Sea, has been studied in terms of the presence of Nematodes of Anisakid family. A total of 150 pieces of fish were purchased in small, medium and large sizes from a Factory in the city of Chabahar. After fish biometry and fish weighing for the presence of Anisakida nematodes، 26 nematodes were isolated from the 15 obdominal area Epinephelus fish. For morphological study, each nematode sample was first clarified with lactophenol. Then it was examined using an optical microscope. After morphological examination of these nematodes, they were first extracted from DNA. Subsequently, using partial primers, a partial cytochrome oxidase genes of 710 bp were introduced into the PCR reaction. Finally, the amplified piece was sequenced .After the sequencing , eight of the nematodes was pseudoterranova, it was identified from the Anisacidae family of nematodes. The parasite was recorded in the gene bank with the accession number of MK317965 and its phylogeny tree was drawn. This nematode was first isolated from the Oeman Sea.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Epinephelus fish, Anisakide, Nematode, pseudoterranova, PCR
Subjects: Q Science > QL Zoology
Depositing User: Mrs najmeh khajeh
Date Deposited: 25 Jun 2019 07:22
Last Modified: 25 Jun 2019 07:22

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