Jalali Ab Bardeh, Shima (2019) Cytotoxic effects study of Artemisia aucheri Boiss on Human Breast Adenocarcinoma Cell Line (MCF-7): Evaluation of Cyclin D1 Expression. Masters thesis, university of zabol.
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Abstract
Breast cancer is the most common invasive tumor and is the second leading cause of death in women. Cyclin D1 is a product of the CCND1 gene, which is located on chromosome 11q13 and reproduces in more than 15% of breast tumors. Artemisia is a herbaceous plant of the family of Composites, due to its antioxidant properties. At the present study, the effect of Artemisia aucheri Boiss on the expression of the Cyclin D1 gene in the human breast cancer cell line (MCF-7) was investigated. The MCF-7 cultured cells were affected by the extract of Artemisia (2, 10, 20, 40, 100, 200, 300, 400, 500, 600 μg / ml) for 24 hours. The MTT assay showed that 100 and 200μg/ml destroyed the MCF-7 cancer cells (50% and >50% respectively). Western blot showed that the expression of the Cyclin D1 protein was decreased after treated with 100 and 200 μg/ml of the extract compared to the control group
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Artemisia aucheri Boiss, Breast cancer , Cyclin D1, MTT assay, Western blot. |
| Subjects: | Q Science > QH Natural history |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 18 Jun 2019 05:37 |
| Last Modified: | 18 Jun 2019 05:37 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2465 |
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