Effect of Foliar Application of Salicylic Acid on Flavone Synthase Gene Expression and Flavonoid compounds in cumin (Cuminum Cyminum) By Real Time PCR

Firoozie, T. (2014) Effect of Foliar Application of Salicylic Acid on Flavone Synthase Gene Expression and Flavonoid compounds in cumin (Cuminum Cyminum) By Real Time PCR. Masters thesis, University of Zabol.

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Cumin (Cuminum Cyminum) is an annual plant of the Apiaceae family which is used in treatment of various diseases such as anticonvulsants, invigorating stomach, diuretic, carminative and indigestion. As a sweating stimulant, Cumin is rich in secondary metabolites. The secondary metabolites are a group of molecules which help the plant to adapt, particularly in case of environmental stresses. Among these compounds are flavonoids and Anthocyanin. Anthocyanins are synthesized by the enzyme activities. Flavones are a subgroup of flavonoids and flavonoids themselves are synthesized in the biosynthesis of flavonoids prior to the flavone. Flavones are also made from flavones in the crotch of Anthocyanidins and pro Anthocyanins. Flavone biosynthesis path is prepared under the effect of two separate enzyme systems, flavone Synthase 1 and flavone Synthase 2, in plants. The role of salicylic acid used as an efficient bio bio-stimulant in improving the biosynthesis of secondary metabolites in plants is known. In this research, his effects of salicylic acid on gene expression of enzymes in the flavonoid flavone of cumin were investigated. The trial was performed at the Center for Environmental Research, University of Zabol (biosynthesis) in the fall, 2013. The plants we were treated re in the vegetative state (21 days) with 1.5 mM salicylic acid and they were harvested at different times of 1, 2, 3, 4 days after application of salicylic acid. Gene expression was analyzed by Real Time PCR and the flavonoid components were measured red both by using Spectrophotometric method and by the Quercetin standard curve. The results showed that compared to the control, the expression of the flavone synthesis gene and the amount of flavonoid components from day 1 to day 3 increased significantly and o y on the third day, the most significant increase was observed, whereas on the fourth day, it experimented a decreasing trend but compared with the control, this trend still indicated a significant increase. In fact, a positive correlation was observe observed between the gene expression of flavonoid Synthase and flavone components, so that the trend of changes of gene expression in different stages of harvest was consistent with the trend of the flavonoid components. In other words, increasing the gene expression increased the amount of flavonoid components and its d decrease showed a reducing process. Therefore, as an inducer, salicylic acid increased the gene expression and flavonoid components in the cumin plant

Item Type: Thesis (Masters)
Uncontrolled Keywords: Salicylic Acid, Gene Expression, Flavonoid Lavonoid Components, Cumin, Flavones , Synthase
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 05 Apr 2016 08:33
Last Modified: 05 Apr 2016 08:33
URI: http://eprints.uoz.ac.ir/id/eprint/245

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