Evaluation of relationship between age and ATF6 expression in Drosophila melanogaster Alzhimer model

Poor Azad, Elahe (2018) Evaluation of relationship between age and ATF6 expression in Drosophila melanogaster Alzhimer model. Masters thesis, University of Zabol.

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Alzheimer’s disease (AD) is described by structural and functional disturbances of the human brain.In AD The amyloid fibril proteins are located and deposited inside neurons as neurofbrillary tangles. These are also deposited extracellular compartment as amyloid plaques.These changes are accompanied by the progressive disorder and loss of nerve cells that are accountable for the loading and managing of information. Finally, AD patients are suffering from loss of and cognitive abilities with intense behavior changes. The aim of this study was to investigate the relationship between expression of ATF6 factor and age in the AD mode of fruit fly (Drosophila melanogaster) induced by Aβ. After mating the virgin female flies GMR-Gal4 and elav-Gal4 with male flies of Human UAS-Aβ42, the first generation were examined. The confirmation of induction of Aβ mutation in first-generation fruit flies was proved by morphological (GMR-Gal4-UAS-Aβ42), behavioral and molecular (elavGal4-UAS-Aβ42) analysis. The first-generation flies were located at 18 °C for first 10 days in order to downregulate the expression of Aβ expression, and then they were located in the normal temperature (24 °C) for next 20 and 30 days in order to evaluate aging process. Then, the analysis of genes expression was performed using Real-time PCR with three replications for all ages at target time points. It was found that the temperature 18 ° C reduced the expression of human Aβ in flies, and the highest toxicity was observed after a decade by placing flies at normal temperature. On the other hand the amount of ATF6 expression was reduced by lowering the temperature and its expression increased in the 20th and 30th days compared to the cold period. In the end, it is suggested that the ATF6 gene will be targeted to develop new therapeutic strategies for AD associated with age. For more information aboute age we need to study in 40 ,50 days drosophila .Also,extension of experiment an analysis various expressions of the elav-Gal4 gene with the aging process in the fruit flies as well as othe important genes involved in the end-stage of UPR 1, such as CHOP 2, Could be also helpfull.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Alzheimer’s disease, ATF6 factor, Amyloid beta, Aging, D. melanogaster
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: Mrs najmeh khajeh
Date Deposited: 06 May 2019 06:48
Last Modified: 06 May 2019 06:48
URI: http://eprints.uoz.ac.ir/id/eprint/2420

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