Mir, Reza (2019) Evaluation of chicken meat contamination with Salmonellaspp. in Zahedan , Iran via PCR. Masters thesis, University of Zabol.
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Abstract
Salmonella bacteria are members of the enterobacteriaceae family, gram negative, aerobic and anaerobic, with a basil shape as large as 0.7-1.5× 2-5 microns and whithout spores and grow normally on normal culture media. Most Salmonella serotypes are potential pathogens for humans and many animals and birds. This bacterium is usually entred orally into the gastrointestinal tract andIt is excreted through human stool or livestock and contaminates water and food and the environment. In addition to food poisoning associated with acute diarrhea and vomiting, salmonella sometimes causes meningitis and septicemia in children. In this study, 150 poultry meat samples were collected from Zahedan township and then, using conventional biochemical methods, the bacteria were identified. The confirmed bacteria were biochemically introduced into the PCR reaction. Four Salmonella bacteria were confirmed by molecular method using invA gene. According to the results, the PCR reaction can be used as a precision method with high specificity to detect Salmonella bacteria in raw products.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Zahedan, Salmonella,Poultry meat, Polymerase Chain Reaction, invA |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 29 Jan 2019 06:24 |
| Last Modified: | 29 Jan 2019 06:24 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2374 |
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