Study of nicotine's effect on α9 receptor, Cyclin D1 and Cytochrome C proteins in MCF-7 cancer cells

Moridi Nejad, S. (2015) Study of nicotine's effect on α9 receptor, Cyclin D1 and Cytochrome C proteins in MCF-7 cancer cells. Masters thesis, university of zabol.

Study of nicotine's effect on α9 receptor.pdf

Download (511kB) | Preview


Background: Breast cancer is the second leading cause of death from cancer in women throughout the world. Nicotine is one of the most important carcinogenic factors in cigarettes, which, by binding to the acetylcholine receptor of the cell, stimulates cell division and increases drug resistance in the cancerous cell of the smoker. In the present study, the effect of nicotine on the expression of α9 receptor gene, Cyclin D1 and Cytochrome C proteins in terms of induction of drug resistance in MCF-7 cancer cell line was investigated. Methods: MCF-7 cells were treated with 100 microliters of nicotine for 72 hours. Then, they were exposed to doxorubicin (1-5-10 μg/ml). Cell survival was evaluated by MTT assay. The biochemical parameters of apoptosis including CyclinD1, Cytochrome C and α9 receptor proteins were assessed by western blot method. α9 receptor expression was investigated using Real-time PCR. Results: The results showed that in nicotine-treated cells, α-9 receptor, and CyclinD1 proteins were increased, however, Cytochrome C was reduced compared with control. There were no significant changes of α9 receptor gene expression in the cells treated with nicotine compared with the control. Conclusion: It is most probably that nicotine in the MCF-7 cell line has an effect on some anti-apoptotic proteins and causing drug resistance and thus inhibits the treatment process.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Breast cancer, nicotinic α9 receptor gene, CyclinD1,Cytochrome Capoptosis, drug resistance
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: Mrs najmeh khajeh
Date Deposited: 29 Dec 2018 10:13
Last Modified: 12 Mar 2019 05:11

Actions (login required)

View Item View Item