Hosseini, Zahra (2018) Survey and comparison of stx1 ,stx2 ,hly and eae frequency in E.coli isolated from crested lark collected in Sistan, South East of Iran. Masters thesis, university of zabol.
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Abstract
Galerida Cristata Sistan can be a carrier of human and animal pathogens, one of the most important of these bacteria is Escherichia coli. This bacteria is a cause of many diseases in humans. The aim of this study was to determine the prevalence of Escherichia coli bacteria in Galerida Cristata Sistan and some genes of stx1, stx2, eae and hly as well as antimicrobial resistance pattern of isolates. A total of 50 swabs were taken from the Galerida Cristata. Separation of the bacteria by culture was performed on differential environments. Antibiotic susceptibility testing was carried out for isolates by disc diffusion method. The presence of genes stx1, stx2, hly and eae in the isolates was evaluated by multiplex PCR method. The results showed that the isolates were resistant to co-amoxyclav antibiotics (100%) and sefetriaxone (100%) and they were sensitive to cefixime. The PCR Multiplex method showed that 33 isolates of Escherichia coli isolated in one isolate (0.5%) with the eae gene showed positive band. The stx1, stx2, and hly genes did not show a positive result
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Galerida Cristata, Escherichia coli,Virulence Factors, Antibiotic Resistance |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 11 Dec 2018 06:09 |
| Last Modified: | 11 Mar 2019 07:11 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2333 |
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