Ghanbari, S. (2018) Molecular, phytochemical, propagation and hair roots induction in Lilium ledebourii Bioss. Doctoral thesis, university of zabol.
|
Text
Molecular, phytochemical, propagation and hair roots induction in Lilium ledebourii.pdf Download (234kB) | Preview |
Abstract
Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. In this study, four tests Molecular, phytochemical, propagation and hair roots induction were done .For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in 3 μM of 2,4-D and 0.5 μM of kinetin. In terms of callus wet weight, the highest amount was found in 3 μM of 2,4-D and 0.5 μM of kinetin. In addition, in terms of diameter, the highest amount was found in 3 μM of 2,4-D, and 0.5 μM of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of 3 μM of 2,4-D, and 0.1 μM of kinetin. In this study, phytochemical compounds were measured quantitatively and qualitatively. Results showed the existence of secondary metabolites in the regenerated plants. Phenolic content was 5.198±0.17 mg in each extract. Radical trapping of the diphenylpicryl hydrazyl test was 3.24±0.52 mg.ml-1. Antioxidant activity based on reduced iron was 25.88±1.47 mmol Fe+2.g-1DW. Flavonoid content of L. ledebourii Bioss was 0.78±0.07 mgRE.G-1. Peroxidase and superoxide dismutase's enzymes activity in L. ledebourii was low. Also, in this study, the combination of saponin, cardiac glycosides, Steroids, Alkaloids and Terpenoid was seen qualitative in the L. ledebourii. In order to study the diversity between lilium masses, ITS regions were used to design the marker. The results showed that the guanine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions of the populations (0.653). Phylogenetic analysis showed that sargentiae and hybrid varieties are older than other varieties of the Lilium family. Also, the location of L. ledebourii varieties (Damash and Namin) was identified in a phylogenetic tree using the ITS marker. Overall, the present research showed that ITS molecular markers are very suitable for phylogenetic studies in the Lilium family
| Item Type: | Thesis (Doctoral) |
|---|---|
| Uncontrolled Keywords: | Hormone, Lilium ledeboorii, Peroxidase, Phylogeny |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 05 Dec 2018 08:13 |
| Last Modified: | 09 Mar 2019 08:47 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2319 |
Actions (login required)
 |
View Item |
