Asadi, Maliheh (2018) Study of oxidative stress and mitochondrial electron transport chain disruptions in neurodegenerative disorders: A Drosophila melanogaster model of Tauopathies. Masters thesis, university of zabol.
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Abstract
Alzheimer's disease is one of the most common forms of Dementias in the world. Alzheimer's disease is diagnosed with the accumulation and phosphorilated of TAU protein in neuron cells. Despite significant advances in the understanding of the mechanisms associated with disease progression, Alzheimer's drug is not yet effective. There is an essential need for more effective treatment of the disease. Therefore, accurate recognition of the cellular and molecular mechanisms of the disease can help in the proper diagnosis and effective treatment. In this research, using Drosophila melanogaster as a genetic model of tauopathies to express the TAU gene simultaneously in the neuronal and glial cells. Larvae olfactory learning assay was carried out to investigate the effect of TAU expression on memory, behavioral tests of NGA and PSA in adult flies. Catalase, superoxide dismutase (SOD), and glutathione (GSH) biochemical tests were evaluated to measure oxidative stress. The Drp1 expression levels were assessed using Real-time PCR. A bad and unhealthy memory was observed in larvae compared with the control the catalase and GSH activities were significantly in the flies than the parent (p≤0.05). In the NGA test, the samples showed a significant decrease in the level of memory and a significant reduction in the ability to climb upwards. Q-PCR showed that Drp1 expression in all cross-sections of UAS-TauR406W and UAS-TauWT was almost significant (p≤0.05). Most probably, increased simultaneously TAU expressions in neurons and glial leads to an increase in ROS and premature aging due to decreased memory levels, the activity of antioxidants enzymes, Drp1 expression and JAK / STAT pathway reduction.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Tauopathies, Oxidative stress, Neurodegenerative diseases, Drosophila melanogaster, Real Time Polymerase Chain Reaction |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 01 Dec 2018 06:40 |
| Last Modified: | 10 Mar 2019 09:37 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2304 |
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