Rahmani, S. (2018) Morphological and molecular identification of Neoscytalidium dimidiatum isolates and other endophyte fungi dieback in Sistan and Kerman Province. Masters thesis, university of zabol.
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Morphological and molecular identification of Neoscytalidium.pdf Download (61kB) | Preview |
Abstract
In order to identify fungi causing dieback and endophitic fungi, samples were collected from shoot tips of some trees in Sistan and parts of Kerman province. Small pieces of the samples cultivated in Patato Dextrose Agar (PDA) after surface disinfection by sodium hypochlorite. A combination of morphological features including conidiomata (if present), conidiogenous loci, shape, size and septation of conidia and conidiophores have been used to identify the taxa. Extraction of genomic DNA performed based on CTAB method. Then ITS region of the ribosomal DNA amplified using ITS1 and ITS4 primers and PCR products were sequenced. Sequences of taxa compared with other correlate and similar sequences in Gene Bank. With morphological data and nucleotide sequencing of ITS-rDNA region, two species of the genus Neoscytalidium including N. dimidiatum (on Ficus religiosa, Nerium oleander) and N. novaehollandiae (on Morus alba) and three taxa including Curvularia spicifera (on Celtis australis), Cadophora malorum (on Vitis vinifera) and Didymella macrostoma (on Citrus sinensis) were identified as fungi causing dieback. Chaetomium jodhpurense on Olea europaea is introduced as both endophyte and shoot tip parasite. Seven species of the genus Alternaria including A. alternata (on Citrus reticulata, Juglans regia, Prunus dulcis), A. burnsii (on Prunus domestica), A. chlamydospora (on Juglans regia), A. consortialis (on Nerium oleander, Prunus dulcis, Vitis vinifera), A. infectoria (on Buxus sempervirens), A. malorum (on Prunus armeniaca) and A. tenuissima (on Citrus sinensis) and three other taxa including Cladosporium macrocarpum (on Ailanthus altissima), Fusarium proliferatum (on Citrus paradisi, Elaeagnus angustifolia, Prunus persica) and Sarocladium strictum (on Salix aegyptiaca) are identified as endophitic fungi from shoot tips. Curvularia australiensis, Fusarium brachygibbosum and Thielavia microspora are new foe the mycobiota of Iran and are reporting for the first time.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Anamorphic fungi, Taxonomy, Dieback, ITS-rDNA |
| Subjects: | Q Science > QK Botany |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 28 Nov 2018 06:35 |
| Last Modified: | 11 Mar 2019 07:09 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/2301 |
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