Analysis of expression pattern of GLUT-2 gene in Ross 308 strain with high and low growth rate

Dehdashti Marghaki, S. (2017) Analysis of expression pattern of GLUT-2 gene in Ross 308 strain with high and low growth rate. Masters thesis, university of zabol.

Analysis of expression pattern of GLUT-2 gene in Ross 308.pdf

Download (144kB) | Preview


Despite extensive advances in establishment of various poultry lines with different chrecterctics, there is still a significant variation in growth rates among and between commercial strains. Besides, the underlying complex mechanisms those are involved in the regulation of the growth rate within and between chicken populations have not yet adequately understood. In this regard, the intestinal nutrient transporters have shown to be critical for regulation of both nutrient absorbtion as well as growth performance of various types of mammals and birds through time. Therefore, the aim of this study was to comparison of the expression levels GLUT-2 in Rice straw commercial chickens with high and low growth rate. For this purpose, 107 Ross broiler chicken were raised under standard condition for 8 weeks. The chicken’s weights, feed consumption were recorded weekly starting on days 0, 7, 14, 21, 28, 35 and 45 and feed conversion ratio calculated based on the obtained data on growth and feed consumption. At the end of the growth period, two birds from each group were randomly selected, slaughtered and their internal organs including the liver, spleen and intestine excised, weighed and analyzed for weight uniformity. Intestinal Total RNA was isolated using RiboEx (Genall, South Korea) and was converted into cDNA with the Hyper Script RT master mix (GenAll) in accordance with the manufacturer's instructions. Relative expression of GLUT-2 transcripts in the duodenum of exprimental groups was compared by quantitative RT-PCR using b-actin as an internal control. Finally, all data was analysed by analysis of variance, using the JMP software version 11; results were compared between groups using Student's t test. There is a no significant difference between the mean of experimental groups in terms of expression of GLUT-2 gene, immunological index, relative weight of analysied organs and feed convertion ratio (p<0.05). Based on the result of this experiment, none of eveluated parameter could be useful to elucidate the reason of growth variation within analysid population.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Gene expression, GLUT-2, Growth traits, Feed conversion ratio
Subjects: Q Science > QL Zoology
Depositing User: Mrs najmeh khajeh
Date Deposited: 23 Apr 2018 04:53
Last Modified: 09 Mar 2019 06:39

Actions (login required)

View Item View Item