The effect of silver nanoparticle on DNA glycation in presence of 3-β-hydroxy butyrate

ShareMollashahi Khamaky, A. R. (2016) The effect of silver nanoparticle on DNA glycation in presence of 3-β-hydroxy butyrate. Masters thesis, university of zabol.

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Advanced glycation end-products (AGEs) resulting from non-enzymatic glycation are one of the major factors implicated in secondary complications of diabetes. Scientists are focusing on discovering new compounds that may be used as potential AGEs inhibitors without affecting the normal structure and function of biomolecules. In this study, we investigated the effect of Silver (I) nanoparticles (AgNps) on DNA glycation process. Materials and Methods: In this study samples included control DNA, DNA & glucose, DNA + glucose & 3 beta hydroxybutyrate and DNA + glucose + 3 beta hydroxybutyrate & silver nanoparticles. All samples were incubated in sodium phosphate buffer at pH 4.7 and concentration of 200 mM and sodium azide at a concentration of 2.0 percent for 28 days. AgNPs were characterized using UV– visible and fluorescence spectroscopy, Circular dichroism (CD) measurement, Agarose gel electrophoresis and nuclease S1 assay techniques. Strong inhibition of advanced glycation end product formation was observed in the presence of silver nanoparticles. The results of this study suggest that silver nanoparticles are a potent antiglycating agent. In the present study it was showed that glucose could cause massive damage in the DNA structure, and causing the strand break and formation of Amadori DNA and advanced glycation end products. But the use of silver nanoparticles could greatly inhibit the effect of adding glucose. These findings strongly suggest that AgNPs may play a therapeutic role in diabetes-related complications.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Glycation, Silver Nanoparticles, DNA, Advanced glycation end-products (AGEs), Silver.
Subjects: Q Science > QH Natural history > QH426 Genetics
Depositing User: Mrs najmeh khajeh
Date Deposited: 19 May 2018 06:10
Last Modified: 03 Mar 2019 05:06

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